Tetacycline Determination from honey using HPLC Method

Abstract

The antibiotics are chemical substances, used in treatment of some disease in animals. Their use in inadequate quantities creates the residues accumulation in food. The tetracycline, which is a broad spectrum antibiotic used for the treatment of some disease at honeybees, was put in evidence and quantify by HPLC method. We used a HPLC with Gold System computer’s software, Beckman pumps and injector, and determination at 355 nm. After establishment of analyze parameters, 10 honey samples from different areas of Romania were analyzed. A number of 3 samples were determined as positive, containing different quantities of antibiotic.