Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1725
Title: Development of Innovative Molecular Methods for the Detection and the Identification of Pseudomonas spp. in Environmental and Clinical Samples
Authors: Calisti, Cecilia
Ruzzi, Maurizio
Keywords: Pseudomonas species;T-RFLP;Molecular typing;PCR;T-RFLP;16S ribosomal DNA;gyrB gene
Issue Date: 2009
Publisher: UASVM Animal Science and Biotechnologies
Source: Calisti C., Ruzzi, M. 2009. Development of Innovative Molecular Methods for the Detection and the Identification of Pseudomonas spp. in Environmental and Clinical Samples. "Bulletin UASVM Animal Science and Biotechnologies" 66 (1-2)
Abstract: 
We have developed a rapid, reliable and sensitive method to analyze, detect and
trace dissemination of pathogenic and spoilage Pseudomonas species in foods and environments. The molecular identification of Pseudomonas species was achieved using a PCR-based assay with primer sets specific for 16S ribosomal DNA and gyrB genes. This PCR assay was found to provide highly genus-specific detection and could be successfully used to identify Pseudomonas in microbial consortia where these bacteria were not abundant. By coupling the PCR assay for 16S rDNA gene to a T-RFLP technique, identification of Pseudomonas strains at species level could be obtained without cultivation. PCR with a combination of two target sequences (multiplex PCR) appeared to be the optimum choice for discriminating between Pseudomonas and closely related genera.
URI: http://hdl.handle.net/2067/1725
ISSN: 1843-5262
Appears in Collections:DABAC - Archivio della produzione scientifica

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