Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1723
Title: Induction of chromosomal aberrations (unstable and stable) by inhibitors of topoisomerase II, m-AMSA and VP16, using conventional Giemsa staining and chromosome painting techniques.
Authors: Mosesso, Pasquale
Darroudi, Firouz
Van Der Berg, Marco A.
Vermeulen, Sandra S.
Palitti, Fabrizio
Natarajan, Adayapalam T.
Issue Date: 1998
Publisher: Oxford University Press
Source: Mosesso, P. et al. 1998. Induction of chromosomal aberrations (unstable and stable) by inhibitors of topoisomerase II, m-AMSA and VP16, using conventional Giemsa staining and chromosome painting techniques. "Mutagenesis" 13(1): 39-43
Abstract: 
Frequencies of symmetrical and asymmetrical exchange aberrations induced by two inhibitors of topoisomerase II, namely, 4'-(9-acridinylamino) methanesulfon-m-anisidide (m-AMSA) and etoposide (VP16), were estimated in human peripheral blood lymphocytes. The aberrations were scored using conventional Giemsa staining and fluorescence in situ hybridization (FISH) techniques, using chromosome-specific DNA libraries. Stable aberrations (translocations) were detected using two cocktails of DNA libraries specific for three chromosomes, namely 1, 3 and X and 2, 4 and 8, representing approximately 40% of the whole human genome. The frequencies of dicentrics and translocations increased in a dose-dependent manner, however, m-AMSA was found to be a more potent inducer of chromosomal aberrations in comparison with VP16 (at concentrations at which comparable frequencies of aberrations were induced) by 20- to 30-fold. When corrected for DNA content of chromosomes in each cocktail, a higher frequency of translocations with the cocktail consisting of chromosomes 2, 4 and 8 in comparison with 1, 3 and X was evident. The genomic translocation frequency calculated from chromosome painting analysis for m-AMSA exceeded that estimated for dicentrics by approximately 2-fold. However, for VP16 almost equal frequencies of both types of chromosome exchange were found
Description: 
L'articolo è disponibile sul sito dell'editore: http://www.oxfordjournals.org
URI: http://hdl.handle.net/2067/1723
ISSN: 0267-8357
DOI: 10.1093/mutage/13.1.39
Appears in Collections:DABAC - Archivio della produzione scientifica

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