Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1700
Title: Comparative proteomic and transcriptional profiling of a bread wheat cultivar and its derived transgenic line over-expressing a low molecular weight glutenin subunit gene in the endosperm
Authors: Scossa, Federico
Laudencia-Chingcuanco, Debby L.
Anderson, Olin D.
Vensel, William H.
Lafiandra, Domenico
D'Ovidio, Renato
Masci, Stefania
Keywords: Genetically modified crops;Microarray;Seed proteome;Wheat
Issue Date: 2008
Publisher: Wiley-Vch Verlag
Source: Scossa F., et al. 2008. Comparative proteomic and transcriptional profiling of a bread wheat cultivar and its derived transgenic line over-expressing a low molecular weight glutenin subunit gene in the endosperm. "Proteomics" 8(14): 2948-2966
Abstract: 
In this paper, a parallel transcriptional and proteomic comparison of seeds from a transformed bread wheat line that over-expresses a transgenic low molecular weight glutenin subunit gene relative to the corresponding non-transformed genotype, has been carried out.
Proteomic analyses showed that, during seed development, several classes of endosperm proteins were differentially accumulated in the transformed endosperm. As a result of the strong increase in the amount of the transgenic protein, the endogenous glutenin subunit, all sub-classes of gliadins, and metabolic as well as Chloroform/Methanol soluble proteins were diminished in the transgenic genotype.
The differential accumulation detected by proteomic analyses, both in mature and developing seeds, was paralleled by the corresponding changes in transcript levels detected by microarray experiments.
Our results suggest that the most evident effect of the strong over-expression of the transgenic glutenin gene consists in a global compensatory response involving a significant decrease in the amounts of polypeptides belonging to the prolamin superfamily. It is likely that such compensation is a consequence of the diversion of amino acid reserves and translation machinery to the synthesis of the transgenic glutenin subunit.
URI: http://hdl.handle.net/2067/1700
ISSN: 1615-9853
Appears in Collections:DABAC - Archivio della produzione scientifica

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