Please use this identifier to cite or link to this item:
Title: Relationship between spontaneous or radiation-induced apoptosis and telomere shortening in G(0) human lymphocytes
Authors: Belloni, Paola
Latini, Paolo
Palitti, Fabrizio
Keywords: Telomere shortening;Spontaneous andradiation-induced apoptosis;Mitochondrial dysfunction
Issue Date: 2010
Publisher: Elsevier
Source: Belloni P., Latini P., Palitti F. 2010. Relationship between spontaneous or radiation-induced apoptosis and telomere shortening in G(0) human lymphocytes. "Mutation Research. Genetic Toxicology and Environmental Mutagenesis" 701(2): 118-122
To examine the correlation between spontaneous or radiation-induced apoptosis and telomere shortening, G0 human peripheral blood lymphocytes were irradiated with X-rays and analyzed for viability, apoptosis, and telomere length. Part of the lymphocytes was kept under liquid-holding conditions for 48 h, and then loaded onto Ficoll-Paque medium to separate apoptotic (high-density) from normal (normal-density) cells. Then all samples were examined for the same three end-points. To determine whether expression of p53 influences the telomere shortening associated with a spontaneous or radiation-induced apoptotic process, the lymphocytes were also analyzed for expression of p53 at 0 and 48 h recovery times (non-irradiated and irradiated samples) and after 2 weeks in liquid-holding conditions (non-irradiated sample).

After 48 h in liquid-holding, the p53-dependent apoptotic lymphocytes in the irradiated cultures presented shortened telomeres. After a 2-week recovery time, non-irradiated cells showed a p53-dependent spontaneous apoptosis, but no telomere shortening. These results demonstrate that radiation-induced apoptosis correlates with shortened telomeres in G0 human lymphocytes. Spontaneous and radiation-induced apoptosis are dependent on expression of p53. In contrast, p53 may not play an effective role in telomere shortening, because spontaneous apoptosis did not correlate with telomere shortening. As most tumours are compromised with respect to p53 function, our findings on the role of p53 in telomere shortening may prove critical for applying therapeutic modalities in the clinic, and may facilitate the design of agents that selectively disrupt telomere integrity in tumour cells.
L'articolo é disponibile sul sito dell'editore:
ISSN: 0027-5107
Appears in Collections:DABAC - Archivio della produzione scientifica

Files in This Item:
File Description SizeFormat
Telomerishrtfinal.pdf68.4 kBAdobe PDFView/Open
Show full item record

Page view(s)

Last Week
Last month
checked on Oct 23, 2020


checked on Oct 23, 2020

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.