Please use this identifier to cite or link to this item:
|Title:||Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus||Authors:||Scapigliati, Giuseppe
Borrego, Juan J.
|Keywords:||Fish Immunology;Molecular Biology and Cell Biology;Sea bass Dicentrarchus labrax;Betanodavirus VER;PCR array;Lymphocytes;Antibody;Anti-viral Immune defence||Issue Date:||2011||Source:||Scapigliati, G. et al. 2010. Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus. "Fish & Shellfish Immunology" 28(2): 303-311||Abstract:||
Naïve sea bass juveniles (38.4 ± 4.5 g) were intraperitoneally infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6 %. At various intervals, sampling of fish tissues were performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: IFN-1, Mx, IL-1, Cox-2; IL-10, TGF-, TCR, CD4, CD8, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable in “vitro” increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for IFN-1, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF- and IL-10 after boosting was also observed, while the other tested genes did not show any significant variations with respect to mock-treated fish. Overall, our work represents a first comprehensive analysis of cellular and molecular immune parameters in a fish species exposed to a pathogenic virus.
L'articolo è disponibile sul sito dell'editore http://www.sciencedirect.com/
|Appears in Collections:||DISA - Archivio della produzione scientifica|
Show full item record
checked on Dec 4, 2020
checked on Dec 4, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.