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Title: Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus
Authors: Scapigliati, Giuseppe
Buonocore, Francesco
Randelli, Elisa
Casani, Daniela
Meloni, Sabrina
Zarletti, Gianpaolo
Tiberi, M.
Pietretti, D.
Boschi, I.
Manchado, Manuel
Martin-Antonio, B.
Jimenez-Cantizano, R.
Bovo, Giuseppe
Borghesan, Fabio
Lorenzen, Niels
Einer-Jensen, Katja
Adams, S.
Thompson, K.
Alonso, C.
Béjar, Julia
Cano, Irene
Borrego, Juan J.
Alvarez, M.Carmen
Keywords: Fish Immunology;Molecular Biology and Cell Biology;Sea bass Dicentrarchus labrax;Betanodavirus VER;PCR array;Lymphocytes;Antibody;Anti-viral Immune defence
Issue Date: 2011
Source: Scapigliati, G. et al. 2010. Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus. "Fish & Shellfish Immunology" 28(2): 303-311
Naïve sea bass juveniles (38.4 ± 4.5 g) were intraperitoneally infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6 %. At various intervals, sampling of fish tissues were performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: IFN-1, Mx, IL-1, Cox-2; IL-10, TGF-, TCR, CD4, CD8, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable in “vitro” increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for IFN-1, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF- and IL-10 after boosting was also observed, while the other tested genes did not show any significant variations with respect to mock-treated fish. Overall, our work represents a first comprehensive analysis of cellular and molecular immune parameters in a fish species exposed to a pathogenic virus.
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ISSN: 1050-4648
DOI: 10.1016/j.fsi.2009.11.008
Appears in Collections:DISA - Archivio della produzione scientifica

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