Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1674
Title: Effect of storage conditions of blood on radiation-induced chromosomal aberrations and apoptosis in human lymphocytes
Authors: Belloni, Paola
Pepe, Gaetano
Palitti, Fabrizio
Keywords: Apotosis;Blood storage;Chromosomal aberrations;X-rays
Issue Date: 2010
Publisher: Elsevier
Source: Belloni P. Pepe G. Palitti F. 2010. Effect of storage conditions of blood on radiation-induced chromosomal aberrations and apoptosis in human lymphocytes. "Mutation Research" 695(1-2): 35-39
Abstract: 
To evaluate the effect of storage conditions of blood on the direct relationship between radiation-induced
chromosome aberrations and apoptosis in human peripheral blood lymphocytes,whole bloodwas irradi-
atedwith 3Gy X-rays. Directly after irradiation, a sample of bloodwas analyzed for chromosome damage
and proliferation index, after phytohaemagglutinin stimulation and incubation at 37 ◦
C for 56 h. Blood
samples were stored for 48 h at 4 and 20 ◦
C with or without phytohaemagglutinin and analyzed for
cell viability and apoptosis at 0, 24 and 48 h storage time. After 48 h of storage, unstimulated cultures
were stimulated to proliferate. These samples and cultures stimulated immediately before storage were
incubated at 37 ◦
C for 56 h and analyzed for chromosome damage and proliferation index. Metaphases
were examined for the presence of dicentrics, excess acentrics, and rings. Storage at 20 ◦
Cwithout phyto-
haemagglutinin for 48 h increases significantly the yield of apoptosis and decreases significantly the yield
of dicentrics. During 48 h of storage time the presence of phytohaemagglutinin and the temperature of
4 ◦
C protected the irradiated lymphocytes from apoptosis allowing accurate estimation of the real yield
of radiation-induced chromosome damage. Therefore these blood-storage conditions enable analysis in
metaphase and may offer some advantages for biodosimetry of absorbed radiation dose.
Description: 
L'articolo è disponibile sul sito dell'editore: http://www.sciencedirect.com
URI: http://hdl.handle.net/2067/1674
ISSN: ISSN: 0027-5107
Appears in Collections:DABAC - Archivio della produzione scientifica

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