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Title: T cell receptor beta chain from sea bream (Sparus aurata): molecular cloning, expression and modelling of the complexes with MHC class I
Authors: Scala, Viviana
Casani, Daniela
Costantini, Susan
Facchiano, Angelo M.
Mazzini, Massimo
Scapigliati, Giuseppe
Buonocore, Francesco
Keywords: Fish Immunology;Molecular Biology;T-cell antigen receptor;TcRβ;Sparus aurata;Real time PCR;3D structure;Class I MHC;TcRβ/MHC complexes
Issue Date: 2008
Publisher: Elsevier
Source: Randelli, E. et al. 2008. T cell receptor beta chain from sea bream (Sparus aurata): Molecular cloning, expression and modelling of the complexes with MHC class I. "Molecular Immunology" 45(7): 2017-2027
The T cell receptor is a fundamental mediator of the adaptive immune responses, since TR on T cells recognize foreign structures (peptides
derived from processed antigens) bound to the major histocompatibility complex (MHC) on APC cells. In the present study, we report the cloning
of six TRB chains cDNA sequences from gilthead sea bream (Sparus aurata), a fish of high economical impact in South Mediterranean aquaculture.
The V-BETA domains have the canonical features of known teleost and mammalian TR V-BETA domains and have been divided in four different
subgroups. A multiple alignment of the six sea bream TRB chains with other known TRB sequences was assembled and showed the conservation
of the four cysteine residues involved in disulphide bonds and of some amino acids with an important role in the assembly and signalling of the
TR /CD3 complex. Real-time PCR analysis was used to investigate TRB basal expression, that was maximum in the thymus followed by gut,
and TRB in vitro expression after stimulation with LPS or PHA-L at 4 and 24 h (only the 4 h stimulation with LPS gave an significant effect).
Moreover, the 3D structures of sea bream TRB chains and MHC-I were predicted by homology modelling with the final aim to investigate the
interaction surface in the V-BETA/MHC-I complexes.
L'articolo è disponibile sul sito dell'editore
ISSN: 0161-5890
DOI: 10.1016/j.molimm.2007.10.027
Appears in Collections:DISA - Archivio della produzione scientifica

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