Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1659
Title: Reduction of the phenolic components in olive-mill wastewater by an enzymatic treatment and its impact on durum wheat (Triticum durum Desf.) germinability
Authors: Casa, Raffaele
D'Annibale, Alessandro
Pieruccetti, Fabio
Stazi, Silvia Rita
Sermanni Giovannozzi, Giovanni
Lo Cascio, Benedetto
Keywords: Laccase;Wastewater;Phytotoxicity;Germination;Wheat
Issue Date: 2003
Publisher: Elsevier Science
Source: Casa, R. et al. 2003. Reduction of the phenolic components in olive-mill wastewater by an enzymatic treatment and its impact on durum wheat (Triticum durum Desf.) germinability. "Chemosphere" 50(8): 959-966.
Abstract: 
Olive-mill wastewater (OMW), an effluent of olive oil extraction process, is annually produced in huge
amounts in olive growing areas. An interesting option for its disposal is the spreading on agricultural land, pro- vided that phytotoxic effects are neutralized. The objective of the present investigation was to evaluate the potential of an enzyme-based treatment in removing OMW phytotoxicity. To this aim, germinability experiments on durum wheat (Triticum durum Desf. cv. Duilio) were conducted in the presence of different dilutions of raw or enzyme-treated OMW.
OMWtreatment with laccase resulted in a 65% and 86% reduction in total phenols and ortho-diphenols respectively,
due their polymerization as revealed by size-exclusion chromatography. Raw OMW exerted a significant concentration-dependent inhibition on the germinability of durum wheat seeds
which was evident up to a dilution rate of 1:8. When the effluent was treated with a fungal laccase, germinability was increased by 57% at a 1:8 dilution and by 94%
at a 1:2 dilution, as compared to the same dilutions using untreated OMW. The treatment with laccase also decreased the mean germination time by about 1 day as compared to untreated controls. These results show that germinability inhibition due to OMW can be reduced effectively using fungal laccase,
suggesting that phenols are the main determinants of its phytotoxicity.
Description: 
L'articolo é disponibile sul sito dell'editore: http://www.sciencedirect.com
URI: http://hdl.handle.net/2067/1659
Appears in Collections:DABAC - Archivio della produzione scientifica

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