Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1644
Title: Molecular linkage map for an intraspecific recombinant inbred population of durum wheat (Triticum turgidum L. var. durum).
Authors: Nachit, Miloudi M.
Elouafi, Ismahane
Pagnotta, Mario Augusto
El Saleh, A.
Iacono, E.
Labhilili, Mustapha
Asbati, A.
Azrak, M.
Hazzam, Hani
Benscher, David
Khairallah, Mirelle M.
Ribaut, Jean Marcel
Tanzarella, Oronzo A.
Porceddu, Enrico
Sorrells, Mark Earl
Keywords: Durum;RFLP;SSR;AFLP;Seed storage proteins;Genetic linkage mapping
Issue Date: 2001
Publisher: Springer-Verlag
Source: Nachit, M.M. et al. 2001. Molecular linkage map for an intraspecific recombinant inbred population of durum wheat (Triticum turgidum L. var. durum). "Theor Appl Genet" 102: 177-186
Abstract: 
Durum wheat (Triticum turgidum L. var. durum) is an economically and nutritionally important cereal crop in the Mediterranean region. To further our understanding of durum genome organization we constructed a durum linkage map using restriction fragment length polymorphisms (RFLPs), simple sequence repeats (SSRs) known as Gatersleben wheat microsatellites (GWMs), amplified fragment length polymorphisms (AFLPs), and seed storage proteins (SSPs: gliadins and glutenins). A population of 110 F9 recombinant inbred lines (RILs) was derived from an intraspecific cross between two durum cultivars, Jennah Khetifa and Cham 1. The two parents exhibit contrasting traits for resistance to biotic and abiotic stresses and for grain quality. In total, 306 markers have been placed on the linkage map – 138 RFLPs, 26 SSRs, 134 AFLPs, five SSPs, and three known genes (one pyruvate decarboxylase and two lipoxygenases). The map is 3598 cM long, with an average distance between markers of 11.8 cM, and 12.1% of the markers deviated significantly from the expected Mendelian ratio 1:1. The molecular markers were evenly distributed between the A and B genomes. The chromosome with the most markers is 1B (41 markers), followed by 3B and 7B, with 25 markers each. The chromosomes with the fewest markers are 2A (11 markers), 5A (12 markers), and 4B (15 markers). In general, there is a good agreement between the map obtained and the Triticeae linkage consensus maps. This intraspecific map provides a useful tool for marker-assisted selection and map-based breeding for resistance to biotic and abiotic stresses and for improvement of grain quality.
Description: 
L'articolo è disponibile sul sito dell'editore:
http://www.springerlink.com
URI: http://hdl.handle.net/2067/1644
ISSN: 0040-5752
Appears in Collections:DABAC - Archivio della produzione scientifica

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