Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1642
Title: Epigenetic regulation of facultative heterochromatinisation in Planococcus citri via the Me(3)K9H3/HP1/Me(3)K20H4 pathway
Authors: Bongiorni, Silvia
Pasqualini, Barbara
Taranta, Monia
Singh, Prim B.
Prantera, Giorgio 
Keywords: Facultative heterochormatin;Histone modifications;HP1;Genomic imprinting;Epigenetics;Mealybugs
Issue Date: 2007
Publisher: The Company of Biologists
Source: Bongiorni, S. et al. 2007. Epigenetic regulation of facultative heterochromatinisation in Planococcus citri via the Me(3)K9H3/HP1/Me(3)K20H4 pathway. "Journal of Cell Science" 120(6): 1072-1080.
Abstract: 
Using RNA interference (RNAi) we have conducted a functional analysis of the HP1-like chromobox gene pchet2 during embryogenesis of the mealybug Planococcus citri.
Knocking down pchet2 expression results in
decondensation of the male-specific chromocenter that normally arises from the developmentally-regulated facultative heterochromatinisation of the paternal chromosome complement. Together with the disappearance of the chromocenter the staining levels of two associated histone modifications, tri-methylated lysine 9 of histone H3 [Me(3)K9H3] and tri-methylated lysine 20 of histone H4 [Me(3)K20H4], are reduced to undetectable levels. Embryos treated with double-stranded RNA (dsRNA) targeting pchet2 also exhibit chromosome abnormalities, such as aberrant chromosome condensation, and also the presence of metaphases that contain ‘lagging’ chromosomes. We conclude that PCHET2 regulates chromosome behavior during metaphase and is a crucial component of a Me(3)K9H3-HP1-Me(3)K20H4 pathway involved in the facultative heterochromatinisation of the (imprinted) paternal chromosome set.
Description: 
L'articolo è disponibile sul sito dell'editore: http://jcs.biologists.org.
URI: http://hdl.handle.net/2067/1642
ISSN: 0021-9533
Appears in Collections:DABAC - Archivio della produzione scientifica

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