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Title: Epigenetic Marks For Chromosome Imprinting During Spermatogenesis In Coccids
Authors: Bongiorni, Silvia
Pugnali, Margherita
Volpi, Silvia
Bizzaro, Davide
Singh, Prim B.
Prantera, Giorgio 
Keywords: Genomic imprinting;Histone modifications;Spermatogenesis;HP1
Issue Date: 2009
Publisher: Springer Verlag
Source: Bongiorni, S. et al. 2009. Epigenetic Marks For Chromosome Imprinting During Spermatogenesis In Coccids. "Chromosoma" 118(4): 501-512
The establishment of sex-specific epigenetic marks during gametogenesis is one of the key feature of genomic imprinting. By immunocytological analysis, we thoroughly characterized the chromatin remodeling events that take place during gametogenesis in the mealybug Planococcus citri, in which an entire haploid set of (imprinted) chromosomes undergoes facultative heterochromatinization in male embryos. Building on our previous work, we have investigated the interplay of several epigenetic marks in the regulation of this genome-wide phenomenon. We characterized the germline patterns of histone modifications, Me(3)K9H3, Me(2)K9H3, and Me (3)K20H4, and of heterochromatic proteins, PCHET2 (HP1-like) and HP2-like during male and female gametogenesis. We found that at all stages in oogenesis chromatin is devoid of any detectable epigenetic marks. On the other hand, spermatogenesis is accompanied by a complex pattern of redistribution of epigenetic marks from euchromatin to heterochromatin, and vice versa. At the end of spermatogenesis, sperm heads are decorated by all the molecules we tested, except for PCHET2. However, only Me(3)K9H3 and Me(2)K9H3 are still detectable in the male pronucleus. We suggest that the histone H3 lysine 9 methylation is the signal used to establish the malespecific imprinting on the paternal genome, thus allowing it to be distinguished from the maternal genome in the developing embryo.
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ISSN: 0009-5915
Appears in Collections:DABAC - Archivio della produzione scientifica

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