Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1612
Title: Molecular and phylogenetic analysis of MADS-box genes of MIKC type and chromosome location of SEP-lke genes in n wheat (Triticum aestivum L.).
Authors: Paolacci, Anna Rita
Tanzarella, Oronzo A.
Porceddu, Enrico
Varotto, Serena
Ciaffi, Mario
Keywords: MADS-box genes;Geni MADS-box;Gene expression;Espressione genica;Wheat;Frumento
Issue Date: 2007
Publisher: Springer-Verlag
Source: Paolacci A.R., et al. 2007. Molecular and phylogenetic analysis of MADS-box genes of MIKC type and chromosome location of SEP-lke genes in n wheat (Triticum aestivum L.). "Mol Genet Genomics" 278: 689-708
Abstract: 
Transcription factors encoded by MIKC-type
MADS-box genes control many important functions in
plants, including Xower development and morphogenesis.
The cloning and characterization of 45 MIKC-type MADSbox
full-length cDNA sequences of common wheat is
reported in the present paper. Wheat EST databases were
searched by known sequences of MIKC-type genes and
primers were designed for cDNA cloning by RT-PCR.
Full-length cDNAs were obtained by 5 and 3 RACE
extension. Southern analysis showed that three copies of
the MIKC sequences, corresponding to the three homoeologous
genes, were present. This genome organization was
further conWrmed by aneuploid analysis of six SEP-like
genes, each showing three copies located in diVerent homoeologous
chromosomes. Phylogenetic analysis included the
wheat MIKC cDNAs into 11 of the 13 MIKC subclasses
identiWed in plants and corresponding to most genes controlling
the Xoral homeotic functions. The expression patterns
of the cDNAs corresponding to diVerent homeotic
classes was analysed in 18 wheat tissues and Xoral organs
by RT-PCR, real time RT-PCR and northern hybridisation.
Description: 
L'articolo è disponibile sul sito dell'editore:
http://www.springerlink.com
URI: http://hdl.handle.net/2067/1612
ISSN: 16174615
Appears in Collections:DABAC - Archivio della produzione scientifica

Files in This Item:
File Description SizeFormat
MGG MIKC 2007.pdf38.24 kBAdobe PDFView/Open
Show full item record

Page view(s)

1
Last Week
0
Last month
0
checked on Oct 23, 2020

Download(s)

2
checked on Oct 23, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.