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|The Protein Disulfide Isomerase gene family in bread wheat (T. aestivum L.)
Paolacci, Anna Rita
Dhanapal, Arun Prabhu
Tanzarella, Oronzo A.
|Wheat;Protein Disulfide Isomerase;Frumento;Disolfuro isomerasi
|d'Aloisio, E. et al. 2010. The Protein Disulfide Isomerase gene family in bread wheat (T. aestivum L.). "BMC Plant Biology" 10:101
Background: The Protein Disulfide Isomerase (PDI) gene family encodes several PDI and PDI-like proteins containing
thioredoxin domains and controlling diversified metabolic functions, including disulfide bond formation and
isomerisation during protein folding. Genomic, cDNA and promoter sequences of the three homoeologous wheat
genes encoding the "typical" PDI had been cloned and characterized in a previous work. The purpose of present
research was the cloning and characterization of the complete set of genes encoding PDI and PDI like proteins in bread
wheat (Triticum aestivum cv Chinese Spring) and the comparison of their sequence, structure and expression with
homologous genes from other plant species.
Results: Eight new non-homoeologous wheat genes were cloned and characterized. The nine PDI and PDI-like
sequences of wheat were located in chromosome regions syntenic to those in rice and assigned to eight plant
phylogenetic groups. The nine wheat genes differed in their sequences, genomic organization as well as in the domain
composition and architecture of their deduced proteins; conversely each of them showed high structural conservation
with genes from other plant species in the same phylogenetic group. The extensive quantitative RT-PCR analysis of the
nine genes in a set of 23 wheat samples, including tissues and developmental stages, showed their constitutive, even
though highly variable expression.
Conclusions: The nine wheat genes showed high diversity, while the members of each phylogenetic group were
highly conserved even between taxonomically distant plant species like the moss Physcomitrella patens. Although
constitutively expressed the nine wheat genes were characterized by different expression profiles reflecting their
different genomic organization, protein domain architecture and probably promoter sequences; the high conservation
among species indicated the ancient origin and diversification of the still evolving gene family. The comprehensive
structural and expression characterization of the complete set of PDI and PDI-like wheat genes represents a basis for
the functional characterization of this gene family in the hexaploid context of bread wheat.
L'articolo é disponibile sul sito dell'editore: http://www.biomedcentral.com
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|DABAC - Archivio della produzione scientifica
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