Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/1578
DC Field | Value | Language |
---|---|---|
dc.contributor.author | D'Eliseo, Donatella | - |
dc.contributor.author | Pisu, Paola | - |
dc.contributor.author | Romano, Chiara | - |
dc.contributor.author | Tubaro, Andrea | - |
dc.contributor.author | De Nunzio, Cosimo | - |
dc.contributor.author | Morrone, Stefania | - |
dc.contributor.author | Santoni, Angela | - |
dc.contributor.author | Stoppacciaro, Antonella | - |
dc.contributor.author | Velotti, Francesca Romana | - |
dc.date.accessioned | 2011-03-22T22:58:21Z | - |
dc.date.available | 2011-03-22T22:58:21Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | D'Eliseo, D. et al. 2010. Granzyme B is expressed in urothelial carcinoma and promotes cancer cell invasion. "International Journal of Cancer" 127 (6): 1283-1294 | it |
dc.identifier.issn | 0020-7136 | - |
dc.identifier.uri | http://hdl.handle.net/2067/1578 | - |
dc.description | L'articolo è disponibile sul sito dell'editore http://onlinelibrary.wiley.com | it |
dc.description.abstract | Granzyme B (GrB) is a serine proteinase known to be expressed by cytotoxic lymphocytes and to induce, in presence of perforin (Pf), apoptosis in target cells. Recently, GrB expression has been shown (often in absence of Pf) in non-lymphoid cells, but its function is not defined. In this study, we investigated GrB and Pf expression in bladder cancer cell lines and in urothelial carcinoma (UC) tissues by RT-PCR, Western blot, ELISA, immunofluorescence, and immunohistochemistry. We also assessed the function of GrB in UC cells; the in vitro function of GrB was examined by loss-of-function experiments. Our results revealed that GrB is expressed, in absence of Pf, in UC cells. Significant differences were found between GrB expression and both increasing pathological tumor spreading and high grade vs low grade pTa tumors. Notably, GrB in UC tissues was concentrated at the cancer invasion front and was expressed in neoplastic cells undergoing epithelial-mesenchymal transition, a key event in carcinoma invasion. Indeed, GrB-positive cells also expressed Snail, N-cadherin, or were negative for E-cadherin. GrB expressed in tumor cell lines was enzymatically active and capable of vitronectin cleavage, implying extracellular matrix (ECM) remodeling by GrB. Inhibition of GrB activity or Stealth RNA interference-mediated GrB gene silencing dramatically suppressed bladder cancer cell invasion through matrigel. This data provides the first evidence for a role of GrB in promoting cancer cell invasion. Taken together, our findings suggest that GrB, via ECM degradation, contributes to the establishment of the UC invasive phenotype. | it |
dc.description.sponsorship | The research was supported by Tuscia University (ex 60%), La Sapienza University (ex 60%), Italian Ministry of University and Research (MiUR), and Associazione Italiana per la Ricerca sul Cancro (AIRC) grants. | it |
dc.language.iso | en | it |
dc.publisher | Wiley-Blackwell | it |
dc.subject | Tumor invasion | it |
dc.subject | Granzyme B | it |
dc.subject | Urothelial carcinoma | it |
dc.title | Granzyme B is expressed in urothelial carcinoma and promotes cancer cell invasion | it |
dc.type | Article | it |
dc.identifier.doi | 10.1002/ijc.25135 | - |
item.fulltext | With Fulltext | - |
item.openairetype | Article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
Appears in Collections: | DECOS - Archivio della produzione scientifica |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
InternJCancer1578.pdf | 974.33 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
5
41
Last Week
0
0
Last month
1
1
checked on Sep 14, 2023
Page view(s)
181
Last Week
0
0
Last month
4
4
checked on Mar 27, 2024
Download(s)
124
checked on Mar 27, 2024
Google ScholarTM
Check
Altmetric
All documents in the "Unitus Open Access" community are published as open access.
All documents in the community "Prodotti della Ricerca" are restricted access unless otherwise indicated for specific documents