Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1561
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dc.contributor.authorPicchietti, Simona-
dc.contributor.authorBelardinelli, Maria Cristina-
dc.contributor.authorTaddei, Anna Rita-
dc.contributor.authorFausto, Anna Maria-
dc.contributor.authorPellegrino, M.-
dc.contributor.authorRossi, M.-
dc.contributor.authorMaggio, Roberto-
dc.contributor.authorGiorgi, Franco-
dc.date.accessioned2011-03-22T14:54:11Z-
dc.date.available2011-03-22T14:54:11Z-
dc.date.issued2009-
dc.identifier.citationCervia, D. et al. 2009. The thyroid disruptor 1,1,1-trichloro-2,2-bis (p-chlorophenyl) ethane (DDT) prevents internalization of the TSH receptor. "Cell and Tissue Research" 336(1): 31-40it
dc.identifier.issn0302-766X-
dc.identifier.urihttp://hdl.handle.net/2067/1561-
dc.descriptionL'articolo è disponibile sul sito dell'editore http://www.springerlink.com/it
dc.description.abstractThe thyroid-stimulating hormone (TSH) receptor (TSHr) was made specifically fluorescent by insertion of a tetracysteine motif (TSHr-FlAsH) into the C-terminal end and transiently transfected into COS-7 and HeLa cells. The observation that TSH administration caused the intracellular level of cAMP to increase in both TSHr-FlAsH-transfected cell types indicated that the FlAsH binding motif did not alter normal TSHr functioning. When transfected into HeLa cells and stimulated with TSH, the TSHr-FlAsH receptor exhibited a pronounced perinuclear labelling pattern, whereas labelling remained on the cell surface following pre-incubation with 1,1,1-trichloro-2,2-bis(p-chlorophenyl) ethane (DDT). Chinese hamster ovary (CHO)-TSHr cells probed with anti-TSHr antibodies were fluorescent mainly in the proximity of the plasma membrane, with fluorescence being primarily restricted to a juxta-nuclear position when exposed to 10 mU/ml TSH for 1 or 5 min. However, in the presence of DDT, the anti-TSHr fluorescence maintained a peripheral location along the cell plasma membrane, even if CHO-TSHr cells were stimulated with TSH for 1 and 5 min. To verify that DDT acted specifically on the TSHr, CHO cells transfected with the A2a receptor were used as controls. Following a 1-min stimulation with 5’-(N-ethyl-carboxamido)-adenosine, A2a receptors were gradually internalized regardless of the presence of DDT in the culture medium. Finally, immunoelectron microscopy of CHO-TSHr cells showed that a 1-min exposure to TSH sufficed to displace anti-TSHr antibodies tagged with 10- nm gold particles into coated pits and vesicles but that their superficial location was retained along the plasma membrane in the presence of DDT.it
dc.language.isoenit
dc.publisherSpringer Verlagit
dc.subjectThyroid-stimulating hormone receptorit
dc.subjectEndocytosisit
dc.subjectA2a receptorit
dc.subject1,1,1-Trichloro-2,2-bis(p-chlorophenyl) ethaneit
dc.subjectA2a receptorit
dc.subjectChinese hamster ovary cellsit
dc.titleThe thyroid disruptor 1,1,1-trichloro-2,2-bis (p-chlorophenyl) ethane (DDT) prevents internalization of the TSH receptorit
dc.typeArticleit
dc.identifier.doi10.1007/s00441-008-0749-7it
item.fulltextWith Fulltext-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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