Please use this identifier to cite or link to this item:
http://hdl.handle.net/2067/1561
DC Field | Value | Language |
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dc.contributor.author | Picchietti, Simona | - |
dc.contributor.author | Belardinelli, Maria Cristina | - |
dc.contributor.author | Taddei, Anna Rita | - |
dc.contributor.author | Fausto, Anna Maria | - |
dc.contributor.author | Pellegrino, M. | - |
dc.contributor.author | Rossi, M. | - |
dc.contributor.author | Maggio, Roberto | - |
dc.contributor.author | Giorgi, Franco | - |
dc.date.accessioned | 2011-03-22T14:54:11Z | - |
dc.date.available | 2011-03-22T14:54:11Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Cervia, D. et al. 2009. The thyroid disruptor 1,1,1-trichloro-2,2-bis (p-chlorophenyl) ethane (DDT) prevents internalization of the TSH receptor. "Cell and Tissue Research" 336(1): 31-40 | it |
dc.identifier.issn | 0302-766X | - |
dc.identifier.uri | http://hdl.handle.net/2067/1561 | - |
dc.description | L'articolo è disponibile sul sito dell'editore http://www.springerlink.com/ | it |
dc.description.abstract | The thyroid-stimulating hormone (TSH) receptor (TSHr) was made specifically fluorescent by insertion of a tetracysteine motif (TSHr-FlAsH) into the C-terminal end and transiently transfected into COS-7 and HeLa cells. The observation that TSH administration caused the intracellular level of cAMP to increase in both TSHr-FlAsH-transfected cell types indicated that the FlAsH binding motif did not alter normal TSHr functioning. When transfected into HeLa cells and stimulated with TSH, the TSHr-FlAsH receptor exhibited a pronounced perinuclear labelling pattern, whereas labelling remained on the cell surface following pre-incubation with 1,1,1-trichloro-2,2-bis(p-chlorophenyl) ethane (DDT). Chinese hamster ovary (CHO)-TSHr cells probed with anti-TSHr antibodies were fluorescent mainly in the proximity of the plasma membrane, with fluorescence being primarily restricted to a juxta-nuclear position when exposed to 10 mU/ml TSH for 1 or 5 min. However, in the presence of DDT, the anti-TSHr fluorescence maintained a peripheral location along the cell plasma membrane, even if CHO-TSHr cells were stimulated with TSH for 1 and 5 min. To verify that DDT acted specifically on the TSHr, CHO cells transfected with the A2a receptor were used as controls. Following a 1-min stimulation with 5’-(N-ethyl-carboxamido)-adenosine, A2a receptors were gradually internalized regardless of the presence of DDT in the culture medium. Finally, immunoelectron microscopy of CHO-TSHr cells showed that a 1-min exposure to TSH sufficed to displace anti-TSHr antibodies tagged with 10- nm gold particles into coated pits and vesicles but that their superficial location was retained along the plasma membrane in the presence of DDT. | it |
dc.language.iso | en | it |
dc.publisher | Springer Verlag | it |
dc.subject | Thyroid-stimulating hormone receptor | it |
dc.subject | Endocytosis | it |
dc.subject | A2a receptor | it |
dc.subject | 1,1,1-Trichloro-2,2-bis(p-chlorophenyl) ethane | it |
dc.subject | A2a receptor | it |
dc.subject | Chinese hamster ovary cells | it |
dc.title | The thyroid disruptor 1,1,1-trichloro-2,2-bis (p-chlorophenyl) ethane (DDT) prevents internalization of the TSH receptor | it |
dc.type | Article | it |
dc.identifier.doi | 10.1007/s00441-008-0749-7 | it |
item.fulltext | With Fulltext | - |
item.openairetype | Article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
Appears in Collections: | DISA - Archivio della produzione scientifica |
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File | Description | Size | Format | |
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CTR 2009.pdf | 42.56 kB | Adobe PDF | View/Open |
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