Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1557
Title: Effect of dietary alginic acid on juvenile tilapia (Oreochromis niloticus) intestinal microbial balance, intestinal histology and growth performance
Authors: Merrifield, Daniel L.
Harper, Glenn M.
Mustafa, Sanaa
Carnevali, Oliana
Picchietti, Simona
Davies, Simon J.
Keywords: Gut microbiota;DGGE;Alginic acid;Ultra histology;Intestine
Issue Date: 2011
Publisher: Springer Verlag
Source: Merrifield, D. L. et al. 2011. Effect of dietary alginic acid on juvenile tilapia (Oreochromis niloticus) intestinal microbial balance, intestinal histology and growth performance. "Cell and Tissue Research" 344 (1): 135-146.
Abstract: 
The aim of the present study was to assess the
effect of a commercial alginic acid source (Ergosan) on
tilapia Oreochromis niloticus intestinal microbial balance,
intestinal morphology, and growth parameters. Fish were
fed a basal control diet or the basal diet plus a source of
alginic acid (5 gkg-1 Ergosan; Schering-Plough Aquaculture,
UK) for 9 weeks. At the end of the trial, light and
electron microscopy demonstrated that the morphology of
the intestinal tract at the gross and ultra-structural level was
not affected by dietary alginic acid inclusion. Both groups
of fish displayed healthy, normal morphology with no signs
of disease, cell or tissue damage. Intestinal epithelial
leucocyte infiltration was not affected by dietary alginic
acid. Molecular bacterial profiles derived from PCR-DGGE
illustrated highly similar microbial communities (both
within the lumen and associated with the intestinal mucosa)
in the respective treatment groups. Microbial ecological
parameters (e.g. species diversity and richness) also
remained unaffected. Although not significant, trends
towards elevated survival and body protein content were
observed in the alginic acid-fed fish. These results are
suggestive that alginic acid does not adversely impact the
indigenous gastrointestinal microbial balance and subsequently
does not impact upon the epithelial brush border
integrity. Validation of non-detrimental impacts of immunostimulatory
products on gastric microbiota and epithelial
integrity should be pursued in future studies as maintaining
microbial balance and epithelial integrity is essential for
proper gut functionality.
Description: 
L'articolo è disponibile sul sito dell'editore http://www.springerlink.com
URI: http://hdl.handle.net/2067/1557
ISSN: 0302-766X
DOI: 10.1007/s00441-010-1125-y
Appears in Collections:DISA - Archivio della produzione scientifica

Files in This Item:
File Description SizeFormat
CTR 2011.pdf54.52 kBAdobe PDFView/Open
Show full item record

Page view(s)

1
Last Week
1
Last month
0
checked on Oct 30, 2020

Download(s)

3
checked on Oct 30, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.