Please use this identifier to cite or link to this item:
Title: Cross activity of orthologous WRKY transcription factors in wheat and Arabidopsis
Authors: Proietti, Silvia
Bertini, Laura
Van der Ent, Sjoerd
Leon-Reyes, Antonio
Pieterse, Corné
Tucci, Marina
Caporale, Carlo
Caruso, Carla
Keywords: Arabidopsis thaliana;Gene expression;Gene orthology;PR4 genes;Transient expression analysis;Transcription;Regulation;Triticum aestivum
Issue Date: 2011
Publisher: Oxford University Press
Source: Proietti, S. et al. 2011. Cross activity of orthologous WRKY transcription factors in wheat and Arabidopsis. "Journal of Experimental Botany" 62(6): 1975-1990.
WRKY proteins are transcription factors involved in many plant processes including plant responses to pathogens.
Here, the cross activity of TaWRKY78 from the monocot wheat and AtWRKY20 from the dicot Arabidopsis on the
cognate promoters of the orthologous PR4-type genes wPR4e and AtHEL of wheat and Arabidopsis, respectively,
was investigated. In vitro analysis showed the ability of TaWRKY78 to bind a –17/+80 region of the wPR4e promoter,
containing one cis-acting W-box. Moreover, transient expression analysis performed on both TaWRKY78 and
AtWRKY20 showed their ability to recognize the cognate cis-acting elements present in the wPR4e and AtHEL
promoters, respectively. Finally, this paper provides evidence that both transcription factors are able to cross-
regulate the orthologous PR4 genes with an efficiency slightly lower than that exerted on the cognate promoters.
The observation that orthologous genes are subjected to similar transcriptional control by orthologous transcription
factors demonstrates that the terminal stages of signal transduction pathways leading to defence are conserved and
suggests a fundamental role of PR4 genes in plant defence. Moreover, these results corroborate the hypothesis that
gene orthology imply similar gene function and that diversification between monocot and dicot has most likely
occurred after the specialization of WRKY function.
L'articolo è disponibile sul sito dell'editore:
ISSN: 0022-0957
Appears in Collections:DABAC - Archivio della produzione scientifica

Files in This Item:
File Description SizeFormat
J. Exp. Bot.-2011-Proietti-1975-90.pdf199.03 kBAdobe PDFView/Open
Show full item record

Page view(s)

Last Week
Last month
checked on Nov 30, 2020


checked on Nov 30, 2020

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.