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Title: The Hsp72 response in peri-parturient dairy cows: relationships with metabolic and immunological parameters
Authors: Catalani, Elisabetta
Amadori, Massimo
Vitali, Andrea
Bernabucci, Umberto
Nardone, Alessandro
Lacetera, Nicola
Keywords: Hsp72;Peripheral blood mononuclear cells;Linfociti;Metabolism;Metabolismo;Peri-partum;Periparto;Dairy cows;Vacche da latte
Issue Date: 2010
Publisher: Springer
Source: Catalani, E. et al. 2010. The Hsp72 response in peri-parturient dairy cows: relationships with metabolic and immunological parameters. "Cell Stress and Chaperones" 15 (6): 781–790
The study was aimed at assessing whether the
peri-parturient period is associated with changes of intracellular and plasma inducible heat shock proteins (Hsp) 72 kDa molecular weight in dairy cows, and to establish possible relationships between Hsp72, metabolic, and immunological parameters subjected to changes around
calving. The study was carried out on 35 healthy periparturient Holstein cows. Three, two, and one week before the expected calving, and 1, 2, 3, 4, and 5 weeks after
calving, body conditions score (BCS) was measured and blood samples were collected to separate plasma and peripheral blood mononuclear cells (PBMC). Concentrations
of Hsp72 in PBMC and plasma increased sharply after calving. In the post-calving period, BCS and plasma glucose declined, whereas plasma nonesterified fatty acids (NEFA) and tumor necrosis factor-alpha increased. The proliferative
responses of PBMC to lipopolysaccharide (LPS) declined progressively after calving. The percentage of PBMC expressing CD14 receptors and Toll-like receptors (TLR)-4
increased and decreased in the early postpartum period, respectively. Correlation analysis revealed significant positive
relationships between Hsp72 and NEFA, and between PBMC proliferation in response to LPS and the percentage of PBMC expressing TLR-4. Conversely, significant negative relationships were found between LPS-triggered proliferation of
PBMC and both intracellular and plasma Hsp72. Literature data and changes of metabolic and immunological parameters
reported herein authorize a few interpretative hypotheses and encourage further studies aimed at assessing possible cause and effect relationships between changes of PBMC and circulating Hsp72, metabolic, and immune parameters in dairy cows.
L'articolo è disponibile sul sito dell'editore
ISSN: 1355-8145
DOI: 10.1007/s12192-010-0186-x
Appears in Collections:DiPA - Archivio della produzione scientifica

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