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Title: The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus)
Authors: Ferguson, R.M.W.
Merrifield, Daniel L.
Harper, Glenn M.
Rawling, Mark D.
Mustafa, Sanaa
Picchietti, Simona
Balcàzar, Josè Luis
Davies, Simon J.
Keywords: Fish;Health;Histology;PCR-DGGE;Probiotic
Issue Date: 2010
Publisher: Wiley-Blackwell
Source: Ferguson, R.M.W. et al. 2010. The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus). "Journal of Applied Microbiology" 109(3): 851-862
Aim: To assess Pediococcus acidilactici as a dietary supplement for on-growing
red tilapia (Oreochromis niloticus).
Methods and Results: Tilapia were fed either a control diet or control diet
supplemented with Ped. acidilactici at 107 CFU g)1 for 32 days. Ped. acidilactici
colonized the intestinal tract and significantly affected the intestinal microbial
communities. PCR-DGGE revealed direct antagonism of gastric Ped. acidilactici
with an endogenous uncultured bacterium during a period of reverting to
nonsupplemented feeding. Light microscopy revealed that gut integrity and
leucocyte levels were unaffected by Ped. acidilactici; however, blood leucocyte
levels and serum lysozyme activity were elevated after 14-days’ feeding. No
significant improvements in growth performance were observed at the end
of the trial (day 32), but survival was significantly higher in the probiotic
Conclusions: The study demonstrates that oral supplementation of Ped. acidilactici
modulates intestinal bacterial communities in on-growing red tilapia and
also stimulates some aspects of the nonspecific immune response.
Significance and Impact of the study: To our knowledge this is the first study
assessing the effects of probiotics on the gut microbiota of tilapia using
culture-independent methods. Such methods are crucial to understand the
mechanisms which underpin and mediate host benefits.
L'articolo è disponibile sul sito dell'editore
ISSN: 1364-5072
DOI: 10.1111/j.1365-2672.2010.04713.x
Appears in Collections:DISA - Archivio della produzione scientifica

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