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Title: Interleukin-10 expression by real-time PCR and homology modelling analysis in the European sea bass (Dicentrarchus labrax L.)
Authors: Buonocore, Francesco
Randelli, Elisa
Bird, Steve
Secombes, Chris J.
Facchiano, Angelo M.
Costantini, Susan
Scapigliati, Giuseppe
Keywords: Fish Immunology;Molecular Biology;Interleukin-10 (IL-10);European sea bass;Dicentrarchus labrax;Expression analysis;Real-time PCR;3D modelling
Issue Date: 2007
Publisher: Elsevier
Source: Buonocore, F. et al. 2007. Interleukin-10 expression by real-time PCR and homology modelling analysis in the European sea bass (Dicentrarchus labrax L.). "Aquaculture" 270(1-4): 512-522
In this paper we investigate the interleukin-10 (IL-10) expression and 3D modelling of the European sea bass (Dicentrarchus
labrax L.). IL-10 is a regulatory cytokine that has been intensively studied in mammals and has been found mainly involved in the
suppression or deactivation of activated immune responses. The full-length sea bass IL-10 cDNA consists of 1015 bp and is
translated in a predicted molecule of 187 amino acids. A multiple alignment of this peptide with other known sequences showed
the conservation of fundamental IL-10 family characteristics. Expression analysis by real-time PCR in control fish showed a high
basal expression in the head kidney (HK), followed by gut and brain. In vitro treatment of HK leucocytes with LPS showed an
intense increase of IL-10 expression at 4 h and a significant decrease at 24 h, with PHA-L no differences were evidenced. A 3D
model for sea bass IL-10 was obtained by accurate homology procedures and putative interaction residues with the IL-10 receptor
described. The results suggested that sea bass IL-10 could be involved in anti-inflammatory responses, but further experiments are
needed to assess its importance in response to pathogenic agents, vaccinations and immunostimulants.
L'articolo è disponibile sul sito dell'editore
ISSN: 0044-8486
DOI: 10.1016/j.aquaculture.2007.05.040
Appears in Collections:DISA - Archivio della produzione scientifica

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