Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1534
DC FieldValueLanguage
dc.contributor.authorRomano, Nicla-
dc.contributor.authorRossi, Francesca-
dc.contributor.authorAbelli, Luigi-
dc.contributor.authorCaccia, Elisabetta-
dc.contributor.authorPiergentili, Rita-
dc.contributor.authorMastrolia, Lucia-
dc.contributor.authorRandelli, Elisa-
dc.contributor.authorBuonocore, Francesco-
dc.date.accessioned2011-03-22T11:41:20Z-
dc.date.available2011-03-22T11:41:20Z-
dc.date.issued2007-
dc.identifier.citationRomano, N. et al. 2007. Majority of TcRβ+ T-lymphocytes located in the thymus and midgut of the bony fish, Dicentrarchus labrax (L.). "Cell and Tissue Research" 329(3): 479-489it
dc.identifier.issn0302-766X-
dc.identifier.urihttp://hdl.handle.net/2067/1534-
dc.descriptionL'articolo è disponibile sul sito dell'editore http://www.springerlink.comit
dc.description.abstractReal-time polymerase chain reaction (PCR) and in situ hybridization analyses were performed to investigate the occurrence and distribution of T-lymphocytes expressing TcRβ in intestine and lymphoid tissues of the bony fish, Dicentrarchus labrax (sea bass). Immunohistochemistry with the monoclonal antibody DLT15 (pan-T-cell marker) was carried out to compare the cytology, distribution and number of T-cells and TcRβ+ cells in the various sampled lymphoid organs. The highest TcRβ expression was revealed by real-time PCR in the thymus, with high levels also being found in the gut. In the thymus, DLT15+ and TcRβ+ cell populations were concentrated in the cortex and TcRβ+ cells were notably reactive at the cortical-medullary border, suggesting a specialized role of this region in thymocyte selection. The density of DLT15+ T-cells increased from the anterior to posterior intestine, whereas TcRβ+ lymphocytes were more numerous in the middle intestine compared with other segments. The existence, in fish thymus, of a medulla and a cortex comparable with those of mammals is revealed by this study. The concentration of TcRβ+ cells in the sea bass midgut also strongly suggests a special role of this intestinal segment in antigen-specific cellular immunity. The large population of TcRβ-/DLT15+ T-cells in the posterior gut can probably be ascribed to the TcRγδ phenotype fraction.it
dc.language.isoenit
dc.publisherSpringer Verlagit
dc.subjectFish Immunologyit
dc.subjectMolecular Biologyit
dc.subjectT-cellsit
dc.subjectTcRβit
dc.subjectThymusit
dc.subjectIntestineit
dc.subjectSea bassit
dc.subjectDicentrarchus labraxit
dc.titleMajority of TcRβ+ T-lymphocytes located in thymus and midgut of the bony fish, Dicentrarchus labrax (L.)it
dc.typeArticleit
dc.identifier.doi10.1007/s00441-007-0429-zit
item.fulltextWith Fulltext-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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