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Title: Majority of TcRβ+ T-lymphocytes located in thymus and midgut of the bony fish, Dicentrarchus labrax (L.)
Authors: Romano, Nicla
Rossi, Francesca
Abelli, Luigi
Caccia, Elisabetta
Piergentili, Rita
Mastrolia, Lucia
Randelli, Elisa
Buonocore, Francesco
Keywords: Fish Immunology;Molecular Biology;T-cells;TcRβ;Thymus;Intestine;Sea bass;Dicentrarchus labrax
Issue Date: 2007
Publisher: Springer Verlag
Source: Romano, N. et al. 2007. Majority of TcRβ+ T-lymphocytes located in the thymus and midgut of the bony fish, Dicentrarchus labrax (L.). "Cell and Tissue Research" 329(3): 479-489
Real-time polymerase chain reaction (PCR) and
in situ hybridization analyses were performed to investigate
the occurrence and distribution of T-lymphocytes expressing
TcRβ in intestine and lymphoid tissues of the bony fish,
Dicentrarchus labrax (sea bass). Immunohistochemistry
with the monoclonal antibody DLT15 (pan-T-cell marker)
was carried out to compare the cytology, distribution and
number of T-cells and TcRβ+ cells in the various sampled
lymphoid organs. The highest TcRβ expression was
revealed by real-time PCR in the thymus, with high levels
also being found in the gut. In the thymus, DLT15+ and
TcRβ+ cell populations were concentrated in the cortex and
TcRβ+ cells were notably reactive at the cortical-medullary
border, suggesting a specialized role of this region in
thymocyte selection. The density of DLT15+ T-cells increased
from the anterior to posterior intestine, whereas TcRβ+
lymphocytes were more numerous in the middle intestine
compared with other segments. The existence, in fish
thymus, of a medulla and a cortex comparable with those
of mammals is revealed by this study. The concentration of
TcRβ+ cells in the sea bass midgut also strongly suggests a
special role of this intestinal segment in antigen-specific
cellular immunity. The large population of TcRβ-/DLT15+
T-cells in the posterior gut can probably be ascribed to the
TcRγδ phenotype fraction.
L'articolo è disponibile sul sito dell'editore
ISSN: 0302-766X
DOI: 10.1007/s00441-007-0429-z
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