Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1499
Title: Regulation of ferulic catabolic genes in Pseudomonas fluorescens BF13: involvement of a MarR family regulator
Authors: Calisti, Cecilia
Ficca, Anna Grazia
Barghini, Paolo
Ruzzi, Maurizio
Keywords: Ferulic acid catabolism;MarR family regulator;Gene transcription;FerR
Issue Date: 2008
Publisher: Springer-Verlag
Source: Calisti, C. et al. 2008. Regulation of ferulic catabolic genes in Pseudomonas fluorescens BF13: involvement of a MarR family regulator. "Applied Microbiology and Biotechnology" 80 (3): 475-483
Abstract: 
In Pseudomonas fluorescens BF13, the cluster of genes essential for degradation of ferulic to vanillic acid (ech, vdh and fcs) is expressed in ferulic but not in succinic grown cells. In the upstream region, we identified a gene, ferR, encoding a protein homologous to transcriptional regulators of the MarR family. A ferR knockout mutant (BF13–89) showed a 3.5-fold increase in expression of an ech-reporter gene fusion compared with the parent strain in succinic-grown cells, indicating that the ferR gene product negatively regulates expression of the ferulic catabolic operon in P. fluorescens BF13. Consistent with the increased expression of the catabolic genes in the ferR mutant, BF13-89 showed a shorter (relative to its FerR+ parent) lag phase during carbon source shift from succinic to ferulic acid. However, expression of ech-lacZ fusion did not increase in BF13–89 grown in the presence of ferulic acid, indicating that FerR has a second function as
transcriptional activator. Expression of ech-lacZ in a feruloyl-CoA synthetase- eficient strain revealed unambiguously that FerR-mediated activation of the ferulic catabolic operon is dependent on the thioester product of the feruloyl-CoA synthetase reaction.
Description: 
L'articolo è disponibile sul sito dell'editore http://www.springerlink.com
URI: http://hdl.handle.net/2067/1499
ISSN: 0175-7598
DOI: 10.1007/s00253-008-1557-4
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