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Title: Effect of wine inhibitors on free pineapple stem bromelain activity in a model wine system
Authors: Esti, Marco
Benucci, Ilaria
Liburdi, Katia
Garzillo, Anna Maria Vittoria
Keywords: Pineapple stem bromelain;Wine inhibitors;Model wine system
Issue Date: 2011
Publisher: American Chemical Society
Source: Esti M. et al. 2011. Effect of wine inhibitors on free pineapple stem bromelain activity in a model wine system. "Journal of Agricultural and Food Chemistry" 59: 3391-3397
The influence of potential inhibitors, naturally present in wine, on the activity of stem bromelain was investigated in order to evaluate the applicability of this enzyme for protein stabilization in white wine. Bromelain proteolytic activity was tested against a synthetic substrate (Bz-Phe-Val-Arg-pNA) in a model wine system after adding ethanol, sulphur dioxide (SO2), skin, seed, and gallic and ellagic tannins at the average range of their concentration in wine.
All the inhibitors of stem bromelain activity tested turned out to be reversible. Ethanol was a competitive inhibitor with a rather limited effect. Gallic and ellagic tannins have no inhibitory effect on stem bromelain activity, while both seed and skin tannins were uncompetitive inhibitors. The strongest inhibition effect was revealed for sulphur dioxide which was a mixed-type inhibitor for the enzyme activity.
This study provides useful information relative to a future biotechnological application of stem bromelain in winemaking.
L'articolo è disponibile sul sito dell'editore
ISSN: 0021-8561
DOI: 10.1021/jf104919v
Appears in Collections:DECOS - Archivio della produzione scientifica

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