Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/1474
DC FieldValueLanguage
dc.contributor.authorCervia, Davide-
dc.contributor.authorDi Giuseppe, Graziano-
dc.contributor.authorRistori, Chiara-
dc.contributor.authorMartini, Davide-
dc.contributor.authorGambellini, Gabriella-
dc.contributor.authorBagnoli, Paola-
dc.contributor.authorDini, Fernando-
dc.date.accessioned2011-03-21T13:50:20Z-
dc.date.available2011-03-21T13:50:20Z-
dc.date.issued2009-
dc.identifier.citationCervia, D. et al. 2009. The secondary metabolite euplotin c induces apoptosis-like death in the marine ciliated protist euplotes vannus. "Journal of Eukaryotic Microbiology" 56(3): 263-269it
dc.identifier.issn1066-5234-
dc.identifier.urihttp://hdl.handle.net/2067/1474-
dc.descriptionL'articolo è disponibile sul sito dell'editore http://onlinelibrary.wiley.com/it
dc.description.abstractThe sesquiterpenoid euplotin C is a secondary metabolite produced by the ciliated protist Euplotes crassus and provides a mechanism for damping populations of potential competitors. Indeed, E. crassus is virtually resistant to its own product while different non-producer species representing an unbiased sample of the marine, interstitial, ciliate diversity are sensitive. For instance, euplotin C exerts a marked disruption of different homeostatic mechanisms in Euplotes vannus. We demonstrate by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay that euplotin C quickly decreases viability and mitochondrial function of E. vannus with a very high efficacy and at micromolar potency. In addition, euplotin C induces apoptosis in E. vannus as 4,6-diamino-2-phenylindole and Terminal Transferase dUTP Nick End Labeling staining show the rapid condensation and fragmentation of nuclear material in cells treated with euplotin C. These effects occur without detectable permeabilisation or rupture of cell membranes and with no major changes in the overall morphology, although some traits, such as vacuolisation and disorganised microtubules, can be observed by transmission electron microscopy. In particular, E. vannus show profound changes of the mitochondrial ultrastructure. Finally, we also show that caspase activity in E. vannus is increased by euplotin C. These data elucidate the pro-apoptotic role of euplotin C and suggest a mechanism for its impact on natural selection.it
dc.language.isoenit
dc.publisherWiley-Blackwellit
dc.subjectCaspasesit
dc.subjectCiliatesit
dc.subjectMitochondriait
dc.subjectNuclear morphologyit
dc.subjectProgrammed cell deathit
dc.subjectUltrastructureit
dc.titleThe secondary metabolite euplotin c induces apoptosis-like death in the marine ciliated protist euplotes vannusit
dc.typeArticleit
dc.identifier.doi10.1111/j.1550-7408.2009.00396.xit
item.fulltextWith Fulltext-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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