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Title: Action Mechanisms of the Secondary Metabolite Euplotin C: Signaling and Functional Role in Euplotes
Authors: Trielli, Francesca
Cervia, Davide
Di Giuseppe, Graziano
Ristori, Chiara
Kruppel, Thomas
Burlando, Bruno
Guella, Graziano
Viarengo, Aldo
Bagnoli, Paola
Delmonte Corrado, Maria Umberta
Dini, Fernando
Keywords: Action potentials;Cation homeostasis;Ciliated protists;Lysosomes;Marine microorganisms;Oxidative stress;Phagocytosis;Sesquiterpenoids
Issue Date: 2008
Publisher: Wiley-Blackwell
Source: Trielli, F. et al. 2008. Action Mechanisms of the Secondary Metabolite Euplotin C: Signaling and Functional Role in Euplotes. "Journal of Eukaryotic Microbiology" 55 (5): 365-373
Among secondary metabolites, the acetylated hemiacetal sesquiterpene euplotin C has been isolated from the marine, ciliated protist Euplotes crassus, and provides an effective mechanism for reducing populations of potential competitors through its cytotoxic properties. However, intracellular signaling mechanisms and their functional correlates mediating the ecological role of euplotin C are largely unknown. We report here that, in E. vannus (an Euplotes morphospecies which does not produce euplotin C and shares with E. crasssus the same interstitial habitat), euplotin C rapidly increases the intracellular concentration of both Ca2+ and Na+, suggesting a generalized effect of this metabolite on cation transport systems. In addition, euplotin C does not induce oxidative stress, but modulates the electrical properties of E. vannus through an increase of the amplitude of graded action potentials. These events parallel the disassembling of the ciliary structures, the inhibition of cell motility, the occurrence of aberrant cytoplasmic vacuoles, and the rapid inhibition of phagocytic activity. Euplotin C also increases lysosomal pH and decreases lysosomal membrane stability of E. vannus. These results suggest that euplotin C exerts a marked disruption of those homeostatic mechanisms whose efficiency represents the essential prerequisite to face the challenges of the interstitial environmental.
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ISSN: 1066-5234
DOI: 10.1111/j.1550-7408.2008.00335.x
Appears in Collections:DISA - Archivio della produzione scientifica

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