Please use this identifier to cite or link to this item: http://hdl.handle.net/2067/144
Title: Double muscling in Marchigiana beef breed is caused by a stop codon in the third exon of myostatin gene
Authors: Marchitelli, Cinzia
Savarese, Maria Carmela
Crisà, Alessandra
Nardone, Alessandro
Ajmone-Marsan, Paolo
Valentini, Alessio
Issue Date: 2003
Publisher: Springer
Source: Marchitelli, C. et al. 2003. Double muscling in Marchigiana beef breed is caused by a stop codon in the third exon of myostatin gene. "Mammalian Genome" 14 (6): 392-395
Abstract: 
Double muscling is a partially recessive trait present in some beef breeds. It shows a high frequency in some breeds, while in others the frequency is low, and double-muscled individuals are rare. The double muscling is caused by an allelic series of mutations that cause a loss of function of the myostatin gene (GDF8). We describe here a new mutation in the myostatin gene in Marchigiana breed, a typical beef breed of Central Italy, in which rare double-muscling individuals have been described. A PCR product of the third exon was sequenced in subjects phenotypically showing double muscling, and a C > T transversion was discovered that introduces a premature stop codon. The variant found adds to the large series of mutations present in cattle, and particularly to the only two causative of double muscling in the third exon. A PCR-RFLP test is described for the rapid and effective identification of both heterozygous and homozygous subjects. It was applied to a larger survey carried on the same and also in two other beef breeds, Chianina and Romagnola. Further individuals carrying the new variant were found in Marchigiana, but none in the other breeds. The results may be important for a better comprehension of the role of myostatin in muscular development, for commercial use and for the inference of phylogeny of this gene.
Description: 
L'articolo è disponibile sul sito dell'editore http://link.springer.com/journal/335
URI: http://hdl.handle.net/2067/144
ISSN: 0938-8990 (print)
1432-1777 (online)
DOI: 10.1007/s00335-002-2176-5
Appears in Collections:DiPA - Archivio della produzione scientifica

Show full item record

Page view(s)

12
Last Week
0
Last month
1
checked on Nov 24, 2020

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons