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Title: Abundance of mRNA of apolipoprotein B100, apolipoprotein E, and microsomal triglyceride transfer protein in liver from periparturient dairy cows
Authors: Bernabucci, Umberto
Ronchi, Bruno
Basiricò, Loredana
Pirazzi, Daniela
Rueca, Fabrizio
Lacetera, Nicola
Nardone, Alessandro
Keywords: Apolipoprotein B;Apolipoprotein E;mRNA;Liver
Issue Date: 2004
Publisher: American Dairy Science Association
Source: Bernabucci, U. et al. 2004. Abundance of mRNA of apolipoprotein B100, apolipoprotein E, and microsomal triglyceride transfer protein in liver from periparturient dairy cows. "Journal of Dairy Science" 87 (9): 2881-2888
Limited secretion of very low density lipoproteins (VLDL) in dairy cows is strongly related to fatty liver and other metabolic disorders in the early postpartum.
Currently, there is limited information on which roles apolipoprotein B100 (ApoB100), apolipoprotein E (ApoE), and microsomal triglyceride transfer protein (MTP)play in that VLDL limitation. To our knowledge, no
studies have simultaneously measured ApoB100, ApoE, and MTP mRNA in periparturient dairy cows. Therefore, a trial was conducted to assess liver gene expression of these proteins in transition dairy cows and to evaluate the relationships between their expression and metabolic status. Eight multiparous Holstein cows were monitored during the transition period. To evaluate metabolic and nutritional status, body condition score was registered, and plasma indexes of energy metabolism and VLDL were determined from 35 d before to 35 d after calving. Liver biopsies were performed on d −35, 3, and 35 relative to day of calving, and gene
expression of ApoB100, ApoE, andMTPwere determined on liver tissue. Body condition, plasma glucose and VLDL decreased, and plasma NEFA and BHBA increased after calving. Compared with values of d −35, on d 3 after calving the ApoB100 mRNA synthesis was lower, whereas MTP and ApoE mRNA abundance were higher. Negative correlation (r = −0.57) between plasma
NEFA concentration and ApoB100 mRNA abundance, and positive correlation between ApoB100 mRNA abundance and plasma cholesterol (r = 0.65) and plasma albumins (r = 0.52) were detected at 3 d postpartum.
Data on changes of gene expression of the 3 main proteins involved in the regulation of synthesis and secretion of VLDL in the liver suggest that decreased mRNA for ApoB100 may be consistent with decreased synthesis and/or secretion ofVLDL from liver during the periparturient period.
ISSN: Print ISSN: 0022-0302: Online ISSN: 1525-3198
Appears in Collections:DiPA - Archivio della produzione scientifica

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