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|Title:||DINAMICA DI COORTI DI HELICOVERPA ARMIGERA (HÜB.) ALIMENTATE CON POMODORO TRANSGENICO||Other Titles:||Dynamics of HELICOVERPA ARMIGERA (HÜB.) cohorts fed with transgenic tomato||Authors:||Speranza, Stefano
|Keywords:||Nottua del pomodoro;Helicoverpa armigera;Pomodoro;Tomato;Biosaggi;Bioassay;Kunitz;AtCys||Issue Date:||2002||Publisher:||Associazione Italiana per la Protezione delle Piante||Source:||SPERANZA S., DATTILO A.M., CARLINI L., SEVERINI M., PUCCI C. 2002 – Dinamica di coorti di Helicoverpa armigera (Hüb.) alimentate con pomodoro transgenico. II Giornate di Studio “Metodi numerici, statistici e informatici nella difesa delle colture agrarie e delle foreste: ricerca e applicazioni”. Pisa, 20-23 Maggio 2002. Notiziario sulla Protezione delle Piante. 15: 293-300. ISSN 1124-9161.||Series/Report no.:||Notiziario sulla Protezione delle Piante;15 (N.S.)||Abstract:||
From the eggs laid on the same day by lepidopteron noctuid Helicoverpa armigera (Hüb.) females, three cohorts were formed. These were bred in a climatic chamber under the same temperature conditions (T = 27°C ± 1°C) and photoperiod (18 hours of light and 6 hours of darkness) and in different feeding conditions. The first cohort was fed with transgenic tomato leaves (Lycopersicum esculentum L.) for the KTI3 genes (a soy gene that codifies for a Kunitz type tryptic inhibitor), the second with transgenic tomato leaves for the AtCys genes (a Arabidopsis thaliana gene, codifying for a cystatinic inhibitor) and the thrid with non transgenic tomato leaves (control). Three new second generation cohorts were formed with the newborn of first generation adults and bred, until the adult stage, under the same laboratory conditions. The experiment was repeated twice. A comparison between the times that the three first and second generation cohorts reached their different lifestyle stages did not show significant differences (P<0.25); this leads us to exclude the possible sub-lethal effects for the H. armigera cohorts fed with both the transgenic diets. This innovative research of the valuation of the sub-lethal effects of the exogenous molecules in transgenic plants increases the knowledge of the new plant-phytophagan interactions that will occur in nature after the controlled release of the new phytophagan-resistant plants.
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