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Title: AFLP markers for DNA fingerprinting in cattle
Authors: Ajmone-Marsan, Paolo
Valentini, Alessio
Bertoni, Giuseppe
Keywords: PCR;restriction polymorphism;selective amplification
Issue Date: 1997
Publisher: Blackwell Publishing
Source: Ajmone-Marsan P., Valentini A., Cassandro M., Vecchiotti-Antaldi G., Bertoni G. & Kuiper M. (1997) AFLP(TM) markers for DNA fingerprinting in cattle. Animal Genetics 28, 418–26
his work reports on use of the recently described amplified fragment length polymorphism (AFLP) technology for DNA fingerprinting in cattle. The AFLP technology produces molecular markers through the high-stringency polymerase chain reaction (PCR)-amplification of restriction fragments that are ligated to synthetic adapters and amplified using primers, complementary to the adapters, which carry selective nucleotides at their 3' ends. While, for plants, the double digestion of genomic DNA with EcoRI and MseI is suggested, in mammals the enzyme combination EcoRI/TaqI produces clearer and more polymorphic AFLP patterns. In a sample of 47 Italian Holstein genotypes, 16 EcoRI/TaqI primer combinations identified 248 polymorphic bands in a species known for its low level of restriction polymorphism. In spite of the low information content carried by each AFLP polymorphism (average polymorphism information content = 0·31), the number of fragments revealed by each primer combination increased significantly the level of genetic information gained in each experiment. AFLP patterns are reproducible in independent experiments and polymorphic fragments segregate in cattle families according to Mendelian rules.
ISSN: 0268-9146
Appears in Collections:DiPA - Archivio della produzione scientifica

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