Comparative proteome analysis of metabolic proteins from seeds of durum wheat (cv. Svevo) subjected to heat stress.
Author(s)
Date Issued
2010
Type
Article
Abstract
In Central and Southern Italy, where durum wheat represents one of the most widely cultivated crops, grain filling occurs during Spring, a period characterised by sudden increases in temperature.
Wheat grain proteins are classified into albumins, globulins, and prolamins. The non-prolamin fractions include proteins with metabolic activity or structural function.
In order to investigate the consequences of heat stress on the accumulation of non-prolamin proteins in mature durum wheat kernels, the Italian cultivar Svevo was subjected to two thermal regimes
(heat stress vs. control) during grain filling. The 2D patterns of non-prolamin proteins were monitored to identify polypeptides affected by heat stress. This study shows that heat stress alters significantly the durum wheat seed proteome, although the fold changes range only between 1.2 and 2.2. This analysis revealed 132 differentially expressed polypeptides, 47 of which were identified by MALDI TOF and MALDI-TOF-TOF MS and included heat shock proteins, proteins involved in the glycolysis and carbohydrate metabolism, as well as stress related proteins. Many of the heat
induced polypeptides are considered to be allergenic for sensitive individuals.
The differences observed with previously reported data regarding bread wheat may be explained by the absence of the D genome in durum wheat
Additional information
L'articolo è disponibile sul sito dell'editore:
www.interscience.wiley.com
www.interscience.wiley.com
Citation
Laino, P. et al. 2010. Comparative proteome analysis of metabolic proteins from seeds of durum wheat (cv. Svevo) subjected to heat stress. "Proteomics" 10(12):2359-2368
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Checksum (MD5)
36d2865c43a06ce5f3ad133ba9b80ab1
Sponsor(s)
Project “Proteine e geni per la protezione delle piante dagli stress biotici e abiotici (PROTEO-STRESS), MIPAF, Italy - MIUR, "Fondo per il sostegno dei giovani e per
favorire la mobilità degli studenti" in the framework “Valorizzazione dei prodotti tipici
dell’agroalimentare e sicurezza alimentare attraverso nuovi sistemi di caratterizzazione e garanzia
di qualità” - COST Action FA0603 Plant Proteomics.
favorire la mobilità degli studenti" in the framework “Valorizzazione dei prodotti tipici
dell’agroalimentare e sicurezza alimentare attraverso nuovi sistemi di caratterizzazione e garanzia
di qualità” - COST Action FA0603 Plant Proteomics.