HPC-T-Annotator: an HPC tool for de novo transcriptome assembly annotation
Author(s)
Date Issued
2024
Type
article
Volume
25
Issue
1
Start Page
272
Journal
Abstract
The availability of transcriptomic data for species without a reference genome enables the construction of de novo transcriptome assemblies as alternative reference resources from RNA-Seq data. A transcriptome provides direct information about a species' protein-coding genes under specific experimental conditions. The de novo assembly process produces a unigenes file in FASTA format, subsequently targeted for the annotation. Homology-based annotation, a method to infer the function of sequences by estimating similarity with other sequences in a reference database, is a computationally demanding procedure.