Livelli di attività antiproteinasiche in piante transgeniche di pomodoro esprimenti inibitori di proteinasi a serina e a cisteina
Author(s)
Date Issued
2004
Type
Conference Proceedings
Abstract
Tomato plants transgenic for genes coding for protein inhibitors, were tested in order to evaluate their capability to inhibit the commercial proteinase activities as well as those present in the midgut of the phytophagous insect Helicoverpa armigera. Leaf extracts of Pi-IV (gene from soybean coding for a Bowman-Birk-like trypsin inhibitor) transgenic T3 plants (cv. UC-82) are able to inhibit bovine pancreatic trypsin and trypsin-like activity of the phytophagous insect 4 times more than control plant extracts; extracts of KTI3 (gene from soybean coding for a Kunitz-like tripsine inhibitor) transgenic T3 plants (cv. Riogrande) are able to inhibit bovine pancreatic trypsin and trypsin-like activity of the insect 200 times more than control plant extracts; leaf extracts of AtCys (gene from Arabidopsis coding for a cysteine protease inhibitor) transgenic T2 plants (cv. Riogrande) are able to inhibit commercial papain and cysteine-like activity of the insect 4 times more than control plant extracts. On the basis of the data it would be interesting, for both speculative and applicative purposes, to use these transgenic plants in order to verify in vivo the action on the mortality and/or on the reproduction ability of the H. armigera, particularly dangerous to the tomato crop.
Citation
Farisei, F. et al. 2004. Livelli di attività antiproteinasiche in piante transgeniche di pomodoro esprimenti inibitori di serin e cistein proteinasi per il controllo di insetti fitofagi dannosi. In: Atti del XIX Congresso nazionale italiano di entomologia (Catania, 10-15 giugno 2002). Sondrio, Tipografia Polaris, pp. 765-769.
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Sponsor(s)
Parzialmente finanziata da finanziamento MURST (Cofin 2002) Prof. Elia Poerio