The face of epicardial and endocardial derived cells in zebrafish
Author(s)
Date Issued
2018
Type
article
Volume
15
Issue
15
Start Page
166
End Page
175
ISSN
144827
Journal
Abstract
Zebrafish hearts can regenerate through activation of growth factors and trans-differentiation of fibroblasts,
epicardial, myocardial and endocardial cells, all positive for GATA4 during the process. A possible model of
regeneration of the whole heart and the regenerating cells in ex-vivo culture is presented here by a stimulation of
cocktail of growth factors. In ex-vivo growth-factors-supplemented culture the heart regeneration was quite
complete without signs of fibrosis. Epicardial- and endocardial-derived cells have been analyzed by electron
microscopy evidencing two main types: 1) larger/prismatic and 2) small/rounded. Type (1) showed on the
surface protein-sculptures, while type(2) was smooth with sparse globular proteins. To confirm their nature we
have contemporarily analyzed their proliferative capability and markers-positivity. The cells treated by growth
factors have at least two-fold more proliferation with GATA4-positivity. The type (1) cell evidenced
WT1+(marker of embryonic epicardium); the type (2) showed NFTA2+(marker of embryonic endocardium);
whereas cTNT-cardiotroponin was negative. Under growth factors stimulation, GATA4+/WT1+ and GATA4+/
NFTA2+ could be suitable candidates to be the cells with capability to move in/out of the tissue, probably by
using their integrins, and it opens the possibility to have long term selected culture to future characterization.
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ECR stem cells 2018.pdf
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Conference(s)
EXPERIMENTAL CELL RESEARCH