Supply of sulphur to S-deficient young barley seedlings restores their capability to cope with iron shortage
Author(s)
Astolfi, Stefania
Zuchi, Sabrina
Hubberten, Hans-Michael
Pinton, Roberto
Hoefgen, Rainer
Date Issued
2010
Type
Article
Abstract
The effect of the S nutritional status on a plant’s capability to cope with Fe shortage was studied in solution
cultivation experiments in barley (Hordeum vulgare L. cv. Europa). Barley is a Strategy II plant and responds to Fe
deficiency by secretion of chelating compounds, phytosiderophores (PS). All PS are derived from nicotianamine
whose precursor is methionine. This suggests that a long-term supply of an inadequate amount of S could reduce
a plant’s capability to respond to Fe deficiency by limiting the rate of PS biosynthesis. The responses of barley
(Hordeum vulgare L. cv. Europa) plants grown for 12 d on Fe-free nutrient solutions (NS) containing 0 or 1.2 mM
SO22
4 , was examined after 24 h or 48 h from transfer to NS containing 1.2 mM SO22
4 . After the supply of S was
restored to S-deprived plants, an increase in PS release in root exudates was evident after 24 h of growth in
S-sufficient NS and the increment reached values up to 4-fold higher than the control 48 h after S resupply. When S
was supplied to S-deficient plants, leaf ATPS (EC 2.7.7.4) and OASTL (EC 4.2.99.8) activities exhibited a progressive
recovery. Furthermore, root HvST1 transcript abundance remained high for 48 h following S resupply and
a significant increase in the level of root HvYS1 transcripts was also found after only 24 h of S resupply. Data
support the idea that the extent to which the plant is able to cope with Fe starvation is strongly associated with its
S nutritional status. In particular, our results are indicative that barley plants fully recover their capability to cope
with Fe shortage after the supply of S is restored to S-deficient plants.
Additional information
L'articolo é disponibile sul sito dell'editore: http://www.jxb.oxfordjournals.org
Citation
Astolfi, S. et al. 2010. Supply of sulphur to S-deficient young barley seedlings restores their capability to cope with iron shortage. "Journal of Experimental Botany" 61(3): 799–806
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Sponsor(s)
Research was supported by grants from Italian MIUR-PRIN 2006 and by funding from the Max-Planck-Society.