Analysis of durum wheat germplasm adapted to different climatic conditions
Author(s)
Mondini, Linda
Farina, Anna
Porceddu, Enrico
Pagnotta, Mario Augusto
Date Issued
2010
Type
Article
Abstract
A study of the extent and patterns of microsatellite diversity in 234 genotypes
from Ethiopian durum wheat (Triticum turgidum) landraces was conducted to
identify areas of diversity that could be used as a source of new germplasm
for developing high yielding and stable varieties. Landraces belonging to nine
populations, from three Ethiopian regions [Tigray (T), Gonder (G) and Shewa
(S)] with different climates, were analysed by using 28 simple sequence repeat
(SSR) markers. The level of polymorphism was high and quite consistent
among populations underlining the great diversity existing. The highest level
of diversity was found within populations, about 75.9%, while about 5.3% was
attributed to differences between regions. The level of expected heterozygosity
was on an average, rather high, ranging from 39% to 56%, whereas the
observed heterozygosity was, on an average, limited to 14%. An average of
about five alleles per locus was detected in each population. Nevertheless,
alleles were not equally present in populations as confirmed by the high level
of expected heterozygosity. The polymorphism information content (PIC) for
the markers assessed showed a wide range of values from 0.14 to 0.92. The
likelihood relationships among the nine Ethiopian populations indicated that
thematerial collected in the Gonder region (a wet climate) was genetically more
diverse than the materials from Shewa and Tigray (dryer climates). The high
number of loci in linkage disequilibrium (LD), up to 23, has demonstrated that
the loci were associated irrespective of their physical location. This holds true
even if the loci are located on different chromosome arms. Genetic diversity
values between populations was very different and was used to produce a
dendrogram showing population relationships.
Additional information
L'articolo è disponibile sul sito dell'editore:http://www.onlinelibrary.wiley.com
Citation
Mondini, L. et al. 2010. Analysis of durum wheat germplasm adapted to different climatic conditions. "Annals of Applied Biology"
156(2): 211–219
156(2): 211–219
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