Molecular linkage map in an intraspecific recombinant inbred population of durum wheat (Triticum turgidum L. var. durum)
Author(s)
Yousefi Javan, Iman
Date Issued
April 20, 2012
Type
Doctoral Thesis
Abstract
A genetic linkage map of tetraploid wheat was constructed based on a cross between two durum
wheat genotypes. The first parental line was a Triticum turgidum ssp. durum (Desf.) genotype
derived from a cross between two durum wheat varieties: Cham 1 and Jennah Ketifa. The second
parental line was a durum wheat genotype derived from a cross between the Triticum turgidum ssp.
Durum, Omrabi 5 and a genotype of wild emmer wheat [T. turgidum ssp. dicoccoides (Körn.)
Thell.].
One hundred ninety-two F8 recombinant inbred lines (RILs) derived from the above mentioned
cross by single-seed descent. A total of 254 markers have been analyzed, including 216
microsatellites and 38 SNPs markers. Linkage analysis defined 14 linkage groups. Most markers
(57.2%) were mapped to the A genome, with an average of 12 markers per chromosome. The
remaining (42.7%) was mapped to the B genome. To construct a stabilized (skeleton) map, markers
interfering with map stability were removed. The skeleton map consisted of 100 markers with a
total length of 3170.29 cM and an average distance of 31.7 cM between adjacent markers. The
length of individual chromosomes ranged between 10.79 cM for chromosome 4B to 58.36 cM for
chromosome 6B. The majority of the markers have a statistical significantly Mendelian segregation
with 1:1 ratio (α = 0.01). A highest percentage of markers resulted similar with the first parental.
Precisely in the chromosomes of (2B, 3A, 5A, 5B).
This SSR and SNP markers showed a high proportion of clustering, which may be indicative of
gene-rich regions. Some of the SSR, SNP markers were mapped for the first time on the current
map, and they had association with some complex traits.
This map provides a useful groundwork for further genetic analyses of important quantitative traits,
positional cloning, and marker-assisted selection, as well as for genome comparative genomics and
genome organization studies in wheat and other cereals.
Additional information
Dottorato di ricerca in Biotecnologie vegetali
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