An oral chitosan DNA vaccine against nodavirus improves transcription of cell-mediated cytotoxicity and interferon genes in European sea bass juveniles gut and survival upon infection
Author(s)
Date Issued
2016
Type
article
Issue
65
Start Page
64
End Page
72
ISSN
145305
Abstract
Vaccines for fish need to be improved for the aquaculture sector, with DNA vaccines and the oral
administration route providing the most promising improvements. In this study, we have created an oral
chitosan-encapsulated DNA vaccine (CP-pNNV) for the nodavirus (NNV) in order to protect the very
susceptible European sea bass (Dicentrarchus labrax). Our data show that the oral CP-pNNV vaccine failed
to induce serum circulating or neutralizing specific antibodies (immunoglobulin M) or to up-regulate
their gene expression in the posterior gut. However, the vaccine up-regulated the expression of genes
related to the cell-mediated cytotoxicity (CMC; tcrb and cd8a) and the interferon pathway (IFN; ifn, mx
and ifng). In addition, 3 months after vaccination, challenged fish showed a retarded onset of fish death
and lower cumulative mortality with a relative survival of 45%. Thus, we created a chitosan-encapsulated
DNA vaccine against NNV that is partly protective to European sea bass juveniles and up-regulates the
transcription of genes related to CMC and IFN. However, further studies are needed to improve the anti-
NNV vaccine and to understand its mechanisms.
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Checksum (MD5)
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Conference(s)
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY