Intestinal T cells of Dicentrarchus labrax (L.): gene expression and functional studies
Author(s)
Date Issued
2011
Type
Article
Abstract
Cellular and molecular data have evidenced a gut-associated lymphoid tissue in a variety of teleost species, abundantly containing T cells, whose origin, selection and functions are still unclear. This study reports CD4, CD8-α, MHCI-α, MHCII-β, rag-1 and TCR-β gene transcription along the intestine (anterior, middle and posterior segments) and in the thymus of one year-old Dicentrarchus labrax (L.).
Real-time PCR findings depicted a main role of the thymus in T cell development, but also rag-1 and CD8-α transcripts are detected in the intestine, having significant expression in the posterior segment. In the whole intestine TCR-β and CD8-α exceeded CD4 transcripts. RNA ISH confirmed these data and detailed that mucosal CD8-α+ cells were especially numerous in the epithelium and in aggregates in the lamina propria. Regional differences in T cell-specific gene expressions are first described in the intestine of a bony fish.
High non-specific cytotoxic activity against xenogeneic and allogeneic cells was found in lymphocytes purified from the intestinal mucosa, providing further insight into their local defence roles.
Additional information
L'articolo è disponibile sul sito dell'editore http://www.sciencedirect.com/
Citation
Picchietti, S. et al. 2011. Intestinal T cells of Dicentrarchus labrax (L.): gene expression and functional studies. "Fish & Shellfish Immunology" 30 (2): 609-617
File(s)![Thumbnail Image]()
Name
IntTcells.doc
Size
30 KB
Format
Microsoft Word
Checksum (MD5)
58814e924a9fe69e0e235372e8b0a855