Chromosome radiosensitivity in human G2 lymphocytes and cell cycle progression
Author(s)
Date Issued
1999
Type
Article
Abstract
PURPOSE: To investigate the possibility that the differential G2-phase radiosensitivity of human peripheral blood lymphocytes, found in normal individuals using the 'G2-phase chromosome radiosensitivity assay', could be attributed to heterogeneity in cellular progression to mitosis rather than differences in radiosensitivity.
MATERIALS AND METHODS: Human peripheral blood lymphocytes, from four different donors, were exposed to 50 cGy X-rays and sampled at different times. The progression of cells into mitosis was monitored by 5-bromo 2'-deoxyuridine (BrdUrd) incorporation.
RESULTS: The heterogeneous G2-phase chromosome radiosensitivity among different donors was abolished when homogeneous G2-phase cell populations were scored; they contained similar frequencies of cells in early or late G2-phase.
CONCLUSIONS: The heterogeneous G2-phase chromosome radiosensitivity, usually found in different normal donors, is caused by the analysis of different cell populations rather than reflecting intrinsic differences in radiosensitivity
Additional information
L'articolo è disponibile sul sito dell'editore: http://informahealthcare.com
Citation
Palitti, F. et al. 1999. Chromosome radiosensitivity in human G2 lymphocytes and cell cycle progression. "International Journal of Radiation Biology" 75(5): 621-627
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