Comparative Proteomic Analysis of Hemocyanins in Dinocras cephalotes and Perla marginata (Plecoptera)
Author(s)
Fochetti, Romolo
Amore, Valentina
Ubero-Pascal, Nicolás
Egidi, Maria Giulia
Timperio, Anna Maria
Date Issued
2011
Type
Article
Abstract
Hemocyanins are large oligomeric respiratory proteins found in many arthropods and
mollusks. The overall expression of hemocyanin mRNA, revealed by studies on Plecoptera hemocyanin
sequencing, has raised the question of whether the protein is expressed or not. In fact, the presence of
expressed hemocyanin has only been reported in the literature for one species, Perla marginata (Panzer,
1799). In this paper, we report the presence of hemocyanin and hexamerin proteins in Dinocras cephalotes
(Curtis, 1827), a species closely related to P. marginata. To assess the presence of hemocyanin, we used
a reproducible and highly sensitive method based on liquid chromatography tandem mass spectrometry.
We conclude that regardless of its putative function (respiratory, immune defense, storage protein), the
hemocyanin is actually expressed in species in which its mRNA is present.
Additional information
L'articolo è disponibile sul sito dell'editore http://www.entsoc.org
Citation
Fochetti, R. et al. 2011. Comparative Proteomic Analysis of Hemocyanins in Dinocras
cephalotes and Perla marginata (Plecoptera). "Environmental entomology" 40(1): 167-171
cephalotes and Perla marginata (Plecoptera). "Environmental entomology" 40(1): 167-171
Subjects
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