The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus)
Author(s)
Date Issued
2010
Type
Article
Abstract
Aim: To assess Pediococcus acidilactici as a dietary supplement for on-growing
red tilapia (Oreochromis niloticus).
Methods and Results: Tilapia were fed either a control diet or control diet
supplemented with Ped. acidilactici at 107 CFU g)1 for 32 days. Ped. acidilactici
colonized the intestinal tract and significantly affected the intestinal microbial
communities. PCR-DGGE revealed direct antagonism of gastric Ped. acidilactici
with an endogenous uncultured bacterium during a period of reverting to
nonsupplemented feeding. Light microscopy revealed that gut integrity and
leucocyte levels were unaffected by Ped. acidilactici; however, blood leucocyte
levels and serum lysozyme activity were elevated after 14-days’ feeding. No
significant improvements in growth performance were observed at the end
of the trial (day 32), but survival was significantly higher in the probiotic
group.
Conclusions: The study demonstrates that oral supplementation of Ped. acidilactici
modulates intestinal bacterial communities in on-growing red tilapia and
also stimulates some aspects of the nonspecific immune response.
Significance and Impact of the study: To our knowledge this is the first study
assessing the effects of probiotics on the gut microbiota of tilapia using
culture-independent methods. Such methods are crucial to understand the
mechanisms which underpin and mediate host benefits.
Additional information
L'articolo è disponibile sul sito dell'editore http://onlinelibrary.wiley.com/
Citation
Ferguson, R.M.W. et al. 2010. The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus). "Journal of Applied Microbiology" 109(3): 851-862
File(s)![Thumbnail Image]()
Name
JAM 2010.pdf
Size
70.67 KB
Format
Adobe PDF
Checksum (MD5)
22567f7b2e8178f7dce0adf40513ef04