Strategie innovative per l'adattamento della bovina da latte a condizioni di caldo ambientale
Author(s)
Primi, Valentina
Date Issued
March 21, 2012
Type
Doctoral Thesis
Abstract
High environmental temperature, affect health, productivity and reproductive efficiency of
dairy cows.
446 Italian Holstein cows were genotyped for two Hsp70.1 single nucleotide polymorphisms
(SNPs): g895 C/- and g1128 G/T in 5’-UTR. 41 cows were selected to represent the different
genotypes. PBMC were isolated from blood samples and heated at 43°C in thermal bath for 1h and
then incubated at 39°C in atmosphere of 5% CO2 for 1, 2, 4, 8, 16 and 24h. Cell viability, gene and
protein expression of Hsp70.1 were determined. Genotypes -- and TG showed higher (P < 0.05)
viability compared with CC and GG, respectively. Genotypes C- and TT had intermediate viability.
Gene expression of Hsp70.1 showed higher (P < 0.001) levels in -- and TG genotype compared with
their counterparts. Genotypes -- and TG showed the higher level of inducible Hsp70.1 protein in
respect to C-, TT and CC, GG. These results indicate that the presence of SNPs (C/- and G/T) in the
5’-UTR region of inducible Hsp-70.1 ameliorates HS response and tolerance to heat of bovine
PBMC.
In the last years, Italian climatic conditions were characterized by an increase of intensity
and frequency of extreme events. To evaluate the heat discomfort on livestock, this study developed
an innovative Italian Forecasting Heat Warning System, using the THI-Temperature Humidity
Index, that is commonly used to quantify the degree of heat stress in farm animals. The system
provides six days forecast Italian maps of THI (day and night time) with indications of four hazard
levels to alert negative effects on production, health and welfare in dairy cows. This system
represents a valuable support to help farm managers.
Additional information
Dottorato di ricerca in Ecologia e gestione delle risorse biologiche
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