Pattern of variation for seed size traits and molecular markers in Italian germplasm of Phaseolus coccineus L. using different molecular markers.
Author(s)
Acampora, Andrea
Ciaffi, Mario
De Pace, Ciro
Paolacci, Anna Rita
Tanzarella, Oronzo A.
Date Issued
2007
Type
Article
Abstract
Variation in Italian germplasm of
Phaseolus coccineus L. was assessed for seed
traits and molecular markers. A total of 130 seeds
and seedlings, five for each of 21 Italian landraces,
an Italian commercial cultivar and four Mesoamerican
landraces of P. coccineus, were analysed
using seven selected PCR markers: three RAPDs,
two ISSRs and two ETs. Seed weight of the
Mesoamerican landraces was £1 g, whereas that
of the Italian landraces varied from 1 g to 2.5 g
and was related to their origin. Oval shape was
more frequent, with round shape observed only in
Mesoamerican landraces. Three seed coat colours
were observed: white, violet mottled or spotted
black and buff spotted brown, also this trait was
related to the origin. The level of polymorphism
detected by molecular markers was low but with
significant discriminant power. ISSRs were the
most effective markers prone to unravel molecular
polymorphism. The within accession component
of variation exceeded that among accessions,
as expected for an allogamous species. However
correct classification of the individuals was
achieved performing either discriminant analysis
of the seed phenotypic traits or cluster analysis of
seedling similarity measure based on the whole
banding patterns obtained by the three marker
types. Our data suggest that the Italian farmers,
starting with ancestral Mesoamerican runner
bean introductions in Europe, bred their own
landraces through selection for seed size and seed
coat colour, but occasional gene flow maintained
variability within landraces bred by different
farmers in the same Italian Region. Selection
favored molecular and seed trait uniformity
within several landraces making them suitable
for certification.
Additional information
L'articolo è disponibile sul sito dell'editore: http://www.springerlink.com
Citation
Acampora A. et al. 2007. Pattern of variation for seed size traits and molecular markers in Italian germplasm of Phaseolus coccineus L. using different molecular markers. "Euphytica" 157(1-2):69-82.
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