Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay
Author(s)
Karim, Latifa
Coppieters, Wouter
Grobet, Luc
Valentini, Alessio
Georges, Michel
Date Issued
2000
Type
Article
Abstract
We herein describe a procedure that allows for simultaneous genotyping of six loss-of-function mutations in the bovine myostatin gene associated with the double-muscling phenotype. The proposed method relies on a multiplex oligonucleotide
ligation assay and detection of the
fluorescently labelled products using automatic sequencers.
Citation
Karim L., Coppieters W., Grobet L., Valentini A. & Georges M. (2000) Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay. Animal Genetics 31, 396–9.
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