Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations
Author(s)
Mendoza-de Gyves, Emilio
Royani, Juwartina Ida
Rugini, Eddo
Date Issued
2007
Type
Article
Abstract
Multiple shoots of high quality were produced in vitro from nodal explants of Tectona grandis. An average of about 4 shoots/uninodal explant was obtained within 4 weeks of culture on Murashige and Skoog’s (mMS) medium modified by 50% reduction in NH4NO3 concentration, supplemented with benzylaminopurine (1.5 mg L−1); indole-3-butyric acid (0.01 mg L−1) and gibberellic acid (0.1 mg L−1). The latter was applied bothin the medium and by soaking the nodal segments for 10 s. in a gibberellic acid solution of 100 mg L−1. Hundred percent of shoots rooted cultured onmodified MS medium containing IBA (0.5 mg L−1) and putrescine (160 mg L−1). Putrescine promoted both strong and highly ramified roots and fastgrowing shoots during the rooting phase, conditioning the plantlets for a good survival and quality. Plantlets were transferred to jiffy pots for a shortacclimatization stage in greenhouse where they survived at 100%. This highly reproducible procedure can be adopted for large scale teak propagation.
Additional information
L'articolo è disponibile sul sito dell'editore http://www.edpsciences.org
Citation
Mendoza-de Gyves E., Royani J. I., Rugini E. 2007. Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations. "Annals of Forest Science" 64(1): 73-78
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