Identification of molecular markers for the characterization of hazelnut oil
Author(s)
Lucchetti, Sabrina
Date Issued
March 29, 2012
Type
Doctoral Thesis
Abstract
In this project it has been set up a simple method for molecular traceability of hazelnut oil through microsatellite markers.
With the aim to achieve the purpose, first of all an amplification/ extraction methodology has been set up on 2 pools of Tonda Gentile Romana hazelnut (TGR). The best technique it is subsequently used to analyze other four hazelnut varieties.
Amplification tests performed on DNAs, extracted through the different methodologies, permitted us to evaluate samples quality and to identify the best PCR conditions for microsatellites analysis.
Study of 9 microsatellites, performed on the best DNAs, has permitted us to define a fingerprint for each variety.
Data obtained from hazelnut varieties compared with a data bank have shown that varieties surely correspond to those declared, except for 2 pools of TGR. The study of two pools with CAC B028 microsatellite has shown that four alleles, instead two, are present in each sample. This result could indicate a non homogeneity of the two matrices.
In TGR pools four alleles instead two have been identified have been identified the study of CAC B028 microsatellite marker has shown that each pool ha four alleles, instead of two, were identified with the CAC B028 marker indicating a possible non homogeneity of the two matrices.
Molecular approach, set up on hazelnut matrix, has shown to be effective for the study of hazelnut oil matrix too, prepared with the two hazelnut pools of TGR. Molecular analysis of oil matrix, in fact, confirm data obtained from hazelnut matrix, include the anomalous result about CAC B028 marker.
To evaluate the homogeneity of one of the TGR pool, analysis on individual hazelnut have been performed, instead on pool, as before. The results show that, for each marker, the global allele number identified in all samples is always more than two expected.
This suggest that the matrix isn’t homogeneous and none individual nut belong to the TGR cultivar.
Additional information
Dottorato di ricerca in Biotecnologie degli alimenti
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