The DNA repair proteins CSA and CSB play a key role in the regulation of cellular division and differentiation
Author(s)
Paccosi, Elena
Date Issued
June 15, 2017
Type
Doctoral Thesis
Abstract
During my PhD research activity I have investigated about Cockayne Syndrome group A and B
proteins role in neural differentiation and cytokinesis control, thus demonstrating that they not only
participate in DNA repair and / or adjust the trigger of apoptosis but also regulate cell fate decision
by taking part in the last step of mitosis, cytokinesis, or cell differentiation.
In my first work I have demonstrated that CSB suppression affects the neuronal differentiation
capability of human neural progenitor cells. Most strikingly, CSB suppression led to a highly
reduced expression of MAP2 (Microtubule-associated Protein 2) accompanied by impaired cell
polarization and neuritogenesis, thus explaining the molecular basis of at least some of the
neurological symptoms reported in CS patients.
Rather, in my second work, I’ve showed a new and previously unpredicted extranuclear role for
both CSA and CSB protein in the abscission control, through the ubiquitination and degradation of
PRC1 (Protein Regulator of Cytokinesis 1) at the midbody.
These findings open a new scenario in the understanding of Cockayne syndrome etiopathology and
of CS proteins role in cell physiology, assigning them a key role in cell fate decisions.
Additional information
Dottorato di ricerca in Ecologia e gestione sostenibile delle risorse ambientali
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