Lipophilization of hydroxytyrosol-enriched fractions from Olea europaea L. by-products and evaluation of the in vitro effects on a model of colorectal cancer cells
Author(s)
Date Issued
2017
Type
article
Volume
65
Issue
31
Start Page
6506
End Page
6512
Abstract
A hydroxytyrosol (HTyr)-enriched fraction containing HTyr 6% w/w, derived from Olea europaea L. byproducts
and obtained using an environmentally and economically sustainable technology, was lipophilized under green chemistry
conditions. The effects of three fractions containing hydroxytyrosyl butanoate, octanoate, and oleate, named, respectively,
lipophilic fractions 5, 6, and 7, and unreacted HTyr on the human colon cancer cell line HCT8-β8 engineered to overexpress
estrogen receptor β (ERβ) were evaluated and compared to those of pure HTyr. The experimental data demonstrated that HTyr
and all fractions showed an antiproliferative effect, as had been observed by the evaluation of the cellular doubling time under
these different conditions (mean control, 32 ± 4 h; HTyr 1, 65 ± 9 h; fraction 5, 64 ± 11 h; fraction 6, 62 ± 14 h; fraction 7, 133
± 30 h). As evidenced, fraction 7 containing hydroxytyrosyl oleate showed the highest activity. These results were related to the
link with ER-β, which was assessed through simultaneous treatment with an inhibitor of ERβ.
Subjects
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